Team:UNITN-Trento/Notebook/Labposts/06/33

From 2013.igem.org

(Difference between revisions)
 
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{
{
"date" : "2013-06-17",
"date" : "2013-06-17",
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"author" : "fabio-bruno",
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"author" : "caterina-michele",
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"title" : "bacillus subtilis promoters (part2)!!",
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"title" : " The Final Transformation!!",
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"content" : "<html> with some tips we selected 2 colonies for each promoter and then put them in LB ( 10 ml) for an overnight incubation (under shake). In the meanwhile we received a new e.coli strain ( NEB5a) and made an inoculum overnight. </html>",
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"content" : " <html>We took the ligation products <a href=\"https://2013.igem.org/Team:UNITN-Trento/Notebook#tn-post-2013-06-14-caterina\"> (see the post) </a>  done by Caterina on Friday and we transformed them in 200 ul of Neb10&beta; competent cells following the protocol. Than we plated them on LB Agar with Cloramphenicol and incubated O/N to see if something will happen. </html>",
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"tags" : "Pveg-PliaG-PlepA"
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"tags" : "PchBA-PchA-PchB"
}
}

Latest revision as of 07:48, 3 October 2013

{ "date" : "2013-06-17", "author" : "caterina-michele", "title" : " The Final Transformation!!", "content" : " We took the ligation products (see the post) done by Caterina on Friday and we transformed them in 200 ul of Neb10β competent cells following the protocol. Than we plated them on LB Agar with Cloramphenicol and incubated O/N to see if something will happen. ", "tags" : "PchBA-PchA-PchB" }

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