Team:UNITN-Trento/Notebook/Labposts/06/49
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(Difference between revisions)
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{ | { | ||
- | "date" : "2013-06- | + | "date" : "2013-06-22", |
- | "author" : " | + | "author" : "bruno", |
- | "title" : " | + | "title" : "Minipreps and screening of Promoters", |
- | "content" : "<html> | + | "content" : "<html>From inocula of the day before I made the minipreps by the following<a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">protocol</a>.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>ng/ul</th></tr><tr><td>K090501</td><td>117,1</td></tr><tr><td>K090504_a</td><td>115,3</td></tr><tr><td>K090504_b</td><td>101,3</td></tr><tr><td>K823000_a</td><td>156,0</td></tr><tr><td>K823000_b</td><td>171,1</td></tr><tr><td>K823000</td><td>115,5</td></tr></table></center></html>}}<html>I made the screening test. To do that we digested 1500 ng of DNA with EcorI and PstI following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">the screening digestion protocol</a>. <br>I used a 1.5% concentration of agarose to create a gel and I used Ethidium bromide instead the normal EuroSafe. In addition I have not used a normal Dye with the 20ul of sample loaded but I used 4ul of 30% glycerol. </html>", |
- | "tags" : " | + | "tags" : "lacOid-PliaG-Pgram+" |
} | } |
Latest revision as of 07:54, 3 October 2013
{ "date" : "2013-06-22", "author" : "bruno", "title" : "Minipreps and screening of Promoters", "content" : "From inocula of the day before I made the minipreps by the followingprotocol.
Quantification results
I made the screening test. To do that we digested 1500 ng of DNA with EcorI and PstI following the screening digestion protocol. Sample | ng/ul |
---|---|
K090501 | 117,1 |
K090504_a | 115,3 |
K090504_b | 101,3 |
K823000_a | 156,0 |
K823000_b | 171,1 |
K823000 | 115,5 |
I used a 1.5% concentration of agarose to create a gel and I used Ethidium bromide instead the normal EuroSafe. In addition I have not used a normal Dye with the 20ul of sample loaded but I used 4ul of 30% glycerol. ", "tags" : "lacOid-PliaG-Pgram+" }