{ "date" : "2013-06-25", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 8)!!", "content" : " today we miniprepped k823001, k823024 and k823026 and screened successfully (1500 ng digested for the promoter, 800 ng for the plasmids). WOOT!! we used a standard gel composition for the backbones and the “ brand new formula” for K823001. Thing is, the 2 plasmids seem to be inverted!!! Now we gotta figure out when the two of them have been inverted!!", "tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac " }