{ "date" : "2013-06-26", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 9)!!", "content" : " in order to verify weather the plasmids have been inverted during miniprep purification protocol, or screening protocol or even before, during plating, we made the minipreps out of the remaining inocula and then screened them: the risults were the same, so probably we made a mistake during plating! Now everything is more clear!!In the afternoon we made the inoculum of the last plasmid ( K823025, sent right from the Part’s Registry Headquarters).", "tags" : " plasmidPxil-plasmidPspac - plasmidluxABCDE " }