Team:UNITN-Trento/Notebook/Labposts/06/64

From 2013.igem.org

(Difference between revisions)
 
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{
{
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"date" : "2013-06-25",
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"date" : "2013-06-27",
"author" : "fabio-bruno",
"author" : "fabio-bruno",
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"title" : "bacillus subtilis promoters and  plasmids (part 8)!!",
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"title" : "bacillus subtilis promoters and  plasmids (part 10)!!",
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"content" : "<html> today we miniprepped k823001, k823024 and k823026 and screened successfully (1500 ng digested for the promoter, 800 ng for the plasmids). WOOT!! we used a standard gel composition for the backbones and the “ brand new formula” for K823001. Thing is, the 2 plasmids seem to be inverted!!! Now we gotta figure out when the two of them have been inverted!!</html>",
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"content" : "<html> I miniprepped K823025 and obtained two good yields: 293,5 ng/ul and 236,6 ng/ul; I screened both samples (1000 ng digested in 50 ul) and the gel confirmed the presence of the plasmid. We have another one!!Afterwards we reported a summary table of part obtained and their yelds</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Summary table|<html><center><table border=\"1\"><tr><th>Sample</th><th>ng/ul</th></tr><tr><td>K823001 a</td><td>436.8</td></tr><tr><td>b</td><td>275.4</td></tr><tr><td>c</td><td>466.5</td></tr><tr><td>K823002a</td><td>138.0</td></tr><tr><td>b</td><td>134.4</td></tr><tr><td>c</td><td>139.7</td></tr><tr><td>K823003 a</td><td>85.4</td></tr><tr><td>b</td><td>99.9</td></tr><tr><td>c</td><td>118.9</td></tr><tr><td>K143012 a</td><td>178.3</td></tr><tr><td>b</td><td>89.2</td></tr><tr><td>c</td><td>121.4</td></tr><tr><td>K823024 a</td><td>362.1</td></tr><tr><td>d</td><td>209.3</td></tr><tr><td>e</td><td>288.2</td></tr><tr><td>K823026 a</td><td>246.3</td></tr><tr><td>b</td><td>315.8</td></tr><tr><td>c</td><td>550.3</td></tr></table></center></html>}}<html></html>",
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"tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac "
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"tags" : "plasmidPxil-plasmidPspac - plasmidluxABCDE-Pveg-PliaG-PlepA-Plial"
}
}

Latest revision as of 07:59, 3 October 2013

{ "date" : "2013-06-27", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 10)!!", "content" : " I miniprepped K823025 and obtained two good yields: 293,5 ng/ul and 236,6 ng/ul; I screened both samples (1000 ng digested in 50 ul) and the gel confirmed the presence of the plasmid. We have another one!!Afterwards we reported a summary table of part obtained and their yelds

Summary table
Sampleng/ul
K823001 a436.8
b275.4
c466.5
K823002a138.0
b134.4
c139.7
K823003 a85.4
b99.9
c118.9
K143012 a178.3
b89.2
c121.4
K823024 a362.1
d209.3
e288.2
K823026 a246.3
b315.8
c550.3
", "tags" : "plasmidPxil-plasmidPspac - plasmidluxABCDE-Pveg-PliaG-PlepA-Plial" }