Team:UNITN-Trento/Notebook/Labposts/06/67

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{ "date" : "2013-06-27", "author" : "gabriele-emil", "title" : "SAMsynthetase and R0010, the neverending story", "content" : "First of all Gabriele quantified the four pSB1C3+R0010 inocula of yesterday.

Quantification
SampleQuantity (ng/µl)
#A243.7
#B194.1
#C245.3
#D252.9

Then, the four quantified samples were screened after a digestion with ExoRI-HF and PstI-HF, with the usual screening protocol (incubated only for 45min, since both the enzymes are HF).

The digestion products were then run on a gel with a transparent loading dye (30% glycerol): each loaded sample contained 16µl of the sample and 4µl of transparent loading dye. Both a 1kb normal ladder and a 100bp transparent ladder were loaded, also the first well was loaded with the usual loading dye.
Gel
Loading scheme
Loading dye
Empty
100bp ladder
#A
#B
#C
#D
Empty
Empty
1kb ladder


Finally, Emil prepared the inocula of the product of ligation of R0010 and SAMsynthetase (in pSB1A2: 6 inocula of the 1:1 ligation product and 3 of the 1:3 ligation producta).", "tags" : "SAMsynthetase-Plac" }