Team:UNITN-Trento/Notebook/Labposts/06/68

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{
{
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"date" : "2013-06-11",
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"date" : "2013-06-28",
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"author" : "thomas-emil-michele",
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"author" : "thomas",
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"title" : "Purification, quantification and digestion of SAMsynthetase and pSB1C3",
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"title" : "Ethylene detection through microGC!!!",
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"content" : "We purified the PCR (third attempt) products of SAMsynthetase and pSB1C3 linearized vector following the Quick Reference protocol. After that, we quantified them using the nanodrop instrument. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>SAM Synthetase</th><th>pSB1C3</th></tr><tr><td>1</td><td>18,6 ng/ul</td><td>21,6 ng/ul</td></tr><tr><td>2</td><td>16,2 ng/ul</td><td>16 ng/ul</td></table></center></html>}}That's definetely not a good result, however we will continue working with them.",
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"content" : "<html>In order to see if our EFE enzyme is effectively active and functional I made a gas-cromatography analysis.<br/> Starting from an overnight culture, I diluted it 1:100 and I incubated it until it reached 0,5 O.D.600. After that, I added 5mM of Arabinose and I incubated the culture at 37&deg;C for about 4-5 hours. In the I connected the vial previously keeped ermetically closed at the micro GC and I took the measure. I did this work for three samples: negative control (not induced), 5mM Arabinose V=1,5ml and 5mM Arabinose V=3ml.  </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Results table|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Ethylene detected</th></tr><tr><td>Not induced</td><td>0&plusmn;15 ppm </td></tr><tr><td>5mM Arabinose V=1,5ml</td><td>30&plusmn;15 ppm </td></tr><tr><td>5mM Arabinose V=3ml</td><td>70&plusmn;15 ppm </td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Apparatus image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/8/8c/Tn-20130628-Apparatus.JPG\" style =\"width: 450px\"></center></html>}}<html>As you can see, seems that the device is correct and functionally active! Definetely a good result!</html>",
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"tags" : "SAMsynthetase"
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"tags" : "EFE"
}
}

Latest revision as of 08:00, 3 October 2013

{ "date" : "2013-06-28", "author" : "thomas", "title" : "Ethylene detection through microGC!!!", "content" : "In order to see if our EFE enzyme is effectively active and functional I made a gas-cromatography analysis.
Starting from an overnight culture, I diluted it 1:100 and I incubated it until it reached 0,5 O.D.600. After that, I added 5mM of Arabinose and I incubated the culture at 37°C for about 4-5 hours. In the I connected the vial previously keeped ermetically closed at the micro GC and I took the measure. I did this work for three samples: negative control (not induced), 5mM Arabinose V=1,5ml and 5mM Arabinose V=3ml.

Results table
SampleEthylene detected
Not induced0±15 ppm
5mM Arabinose V=1,5ml30±15 ppm
5mM Arabinose V=3ml70±15 ppm
Apparatus image
As you can see, seems that the device is correct and functionally active! Definetely a good result!", "tags" : "EFE" }

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