Revision as of 08:00, 3 October 2013 by Ggirelli (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

{ "date" : "2013-06-28", "author" : "thomas", "title" : "Ethylene detection through microGC!!!", "content" : "In order to see if our EFE enzyme is effectively active and functional I made a gas-cromatography analysis.
Starting from an overnight culture, I diluted it 1:100 and I incubated it until it reached 0,5 O.D.600. After that, I added 5mM of Arabinose and I incubated the culture at 37°C for about 4-5 hours. In the I connected the vial previously keeped ermetically closed at the micro GC and I took the measure. I did this work for three samples: negative control (not induced), 5mM Arabinose V=1,5ml and 5mM Arabinose V=3ml.

Results table
SampleEthylene detected
Not induced0±15 ppm
5mM Arabinose V=1,5ml30±15 ppm
5mM Arabinose V=3ml70±15 ppm
As you can see, seems that the device is correct and functionally active! Definetely a good result!", "tags" : "EFE" }