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{"date":"2013-07-01","author":"viola","title":"PCR of the double terminator BBa_B0015","content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:

B0015 in pSB1AK3
219,4 ng/ µl
B0015 in pSB1C3
413,3 ng/ µl
H20 33.3 µl 33.3 µl
DNA 0.5 µl 0.5 µl
DNTPs 1 µl 1 µl
pref. FW 2.5 µl 2.5 µl
suff. RV 2.5 µl 2.5 µl
BUFFER 10 µl 10 µl
Phusion pol. 0.5 µl 0.5 µl
}}</html>\" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.","tags":"EFE"}