Team:UNITN-Trento/Notebook/Labposts/07/05

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(Difference between revisions)

Latest revision as of 10:33, 3 October 2013

{"date" : "2013-07-02","author" : "caterina-michele","title" : "Incredible: E.coli could growth!","content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ","tags" : "PchBA-BMST1-araC-pBAD"}

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+{"date" : "2013-07-02","author" : "caterina-michele","title" : "Incredible: E.coli could growth!","content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ","tags" : "PchBA-BMST1-araC-pBAD"}
-	"date" : "2013-07-22",
-	"author" : "thomas",
-	"title" : "Inocula of AraC-pBAD + EFE + Venus",
-	"content" : "<html>This is the part four of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37&deg;C in thermoshaker.  </html>",
-	"tags" : "EFE-Venus"
-}