Team:UNITN-Trento/Notebook/Labposts/07/07

From 2013.igem.org

(Difference between revisions)
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{
{
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"date" : "2013-07-23",
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"date" : "2013-07-03",
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"author" : "emil",
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"author" : "gabriele",
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"title" : "The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP",
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"title" : "An easy day...",
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"content" : "<html>I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400&micro;l of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates.Afterwards I transformed Neb10&beta; with 100 ng of K823026+E0840 following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a>.</html>",
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"content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-gabriele\">yesterday</a>:<br/><br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th></tr><tr><td>Ctrl</td><td>4</td></tr><tr><td>0.5:1</td><td>2</td></tr><tr><td>0.5:2</td><td>0</td></tr></table></center><br/><hr><br/>Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th colspan=\"4\">Loading scheme</th></tr><tr><td>1kb ladder</td><td>G1</td><td>G2</td><td>G3</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/d/d3/Tn-20130703-GGCM_linearizationpSB1C3_FAILED.jpg\" alt=\"Gel\" width=\"450px\"></center></html>}}<html></html>",
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"tags" : "Bacillus subtilis-pSpac+GFP"
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"tags" : "SAMsynthetase-pSB1C3"
}
}

Revision as of 08:22, 3 October 2013

{ "date" : "2013-07-03", "author" : "gabriele", "title" : "An easy day...", "content" : "First of all I counted the colonies from yesterday:

plate#colonies
Ctrl4
0.5:12
0.5:20



Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.
Gel
Loading scheme
1kb ladderG1G2G3
\"Gel\"
", "tags" : "SAMsynthetase-pSB1C3" }