Team:UNITN-Trento/Notebook/Labposts/07/13

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:35, 3 October 2013

{"date" : "2013-07-04","author" : "thomas","title" : "Ethylene production kinetic assay","content" : "In order to see the progress of the production of ethylene in time, 7 ml of culture were grown until O.D. 600 reached 0,5. The culture was then induced with 70 ul arabinose (5mM) and putted with a stirrer into a sealed tube with a pierceable septum. The tube was then connected to the Micro GC (Agilent 3000A) and immersed in a thermal bath at 37°C with magnetic stirring. A measurment was taken every hour for 8 hours. The results were not so exciting. The colony didn't produce any level ethylene and we have no idea why. Maybe the colony from which the inocula were done wasn't the right one. I'll try the experiment again the next monday!

Apparatus image

","tags" : "EFE"}

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+{"date" : "2013-07-04","author" : "thomas","title" : "Ethylene production kinetic assay","content" : "<html>In order to see the progress of the production of ethylene in time, 7 ml of culture were grown until O.D. 600 reached 0,5. The culture was then induced with 70 ul arabinose (5mM) and putted with a stirrer into a sealed tube with a pierceable septum. The tube was then connected to the Micro GC (Agilent 3000A) and immersed in a thermal bath at 37&deg;C with magnetic stirring. A measurment was taken every hour for 8 hours. The results were not so exciting. The colony didn't produce any level ethylene and we have no idea why. Maybe the colony from which the inocula were done wasn't the right one. I'll try the experiment again the next monday! <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Apparatus image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/9/96/Tn-2013_EFE_kinetic_apparatus.jpg\" style =\"width: 450px\"></center></html>}}<html></html>","tags" : "EFE"}
-	"date" : "2013-07-16",
-	"author" : "fabio-bruno",
-	"title" : " blue and red light sensors!!",
-	"content" : "<html>  the time has come to extract from the 2013 kit some other new parts: they will be needed to allow our divice to be activated by blue or red light!! Awesome, don’t you think? The parts are: <br>K592016: a blue sensor with its response regulator; k592006: a promoter induced by the blue regulator; <br>k519030: the device with the red sensor and necessary enzymes; r0082: the red promoter. <br>We after extracting them we transformed in neb5a and plated. </html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
-}