Team:UNITN-Trento/Notebook/Labposts/07/13

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:35, 3 October 2013

{"date" : "2013-07-04","author" : "thomas","title" : "Ethylene production kinetic assay","content" : "In order to see the progress of the production of ethylene in time, 7 ml of culture were grown until O.D. 600 reached 0,5. The culture was then induced with 70 ul arabinose (5mM) and putted with a stirrer into a sealed tube with a pierceable septum. The tube was then connected to the Micro GC (Agilent 3000A) and immersed in a thermal bath at 37°C with magnetic stirring. A measurment was taken every hour for 8 hours. The results were not so exciting. The colony didn't produce any level ethylene and we have no idea why. Maybe the colony from which the inocula were done wasn't the right one. I'll try the experiment again the next monday!

Apparatus image

","tags" : "EFE"}

@@ Line 1: / Line 1: @@
-{
+{"date" : "2013-07-04","author" : "thomas","title" : "Ethylene production kinetic assay","content" : "<html>In order to see the progress of the production of ethylene in time, 7 ml of culture were grown until O.D. 600 reached 0,5. The culture was then induced with 70 ul arabinose (5mM) and putted with a stirrer into a sealed tube with a pierceable septum. The tube was then connected to the Micro GC (Agilent 3000A) and immersed in a thermal bath at 37&deg;C with magnetic stirring. A measurment was taken every hour for 8 hours. The results were not so exciting. The colony didn't produce any level ethylene and we have no idea why. Maybe the colony from which the inocula were done wasn't the right one. I'll try the experiment again the next monday! <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Apparatus image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/9/96/Tn-2013_EFE_kinetic_apparatus.jpg\" style =\"width: 450px\"></center></html>}}<html></html>","tags" : "EFE"}
-	"date" : "2013-07-05",
-	"author" : "caterina-michele",
-	"title" : "Can I SNIFF?",
-	"content" : " <html> You know, after a BBBQ, everything is more difficult!! In particular today was an exausting day. While in the morning Caterina did three miniprep from the inocula (post del giorno prima metti link = 4 with araCpBAD + BSTM1 in pSB1C3 and 1 only control of Neb10&beta, mettere immagine del gel di cate) Michele took the two inocula that left and did 7 diluition 1:100 in 20 mL of LB. In particular he did 2 diluition for the control and five for the bacteria with the plasmid. He put them at 37°C in agitation waiting for them reaching O.D. = 0.5. After four hours he did the induction with arabinosio even if the bacteria with the plasmid were at about 0.3 O.D. The induction was done only on five samples to have another control. After two hours it was added different concentrations of Salycilic Acid (in solution with H20 and etanol) in different samples and after two more hours we did a SNIFF Test to understand if some Mesa was produced. The results were not so brilliant: in fact the bacteria in which were added higher concentrations of SA stinked a bit less and were more fresh.Durante la giornata cate ha fatto anche pcr per J45119 ma fail (immagine)In più fatte prime misure con GC per vedere detabilità mesa (grafici)  </html>   ",
-	"tags" : "PchBA-BMST1-araC-pBAD"
-}