http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/24&feed=atom&action=historyTeam:UNITN-Trento/Notebook/Labposts/07/24 - Revision history2024-03-28T14:48:31ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/24&diff=274409&oldid=prevGgirelli at 10:37, 3 October 20132013-10-03T10:37:54Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{"date" : "2013-07-<ins class="diffchange diffchange-inline">10</ins>","author" : "<ins class="diffchange diffchange-inline">fabio</ins>","title" : " <ins class="diffchange diffchange-inline">ripenator v1.0 </ins>","content" : "<html> <ins class="diffchange diffchange-inline"> this morning, Bruno is not available, so I will take care of the ripenator. </ins>I <ins class="diffchange diffchange-inline">have </ins>to <ins class="diffchange diffchange-inline">replace all </ins>the <ins class="diffchange diffchange-inline">cultures with new LB and new bacteria </ins>: <ins class="diffchange diffchange-inline">I’m gonna put 300 ml of LB in each beutas, then connect immediately </ins>a <ins class="diffchange diffchange-inline">beuta with LB and 3ml of not-induced bacteria to the control banana!! After that, I will dissolve 78 ul of MESA in the second beuta and connect to the blocked banana! As far as the ethylene sample is concerned, I have to put 3 ml of bacteria in the last beuta, wait for the OD lecture to reach the value of 0,5 and then induce the culture with Arabinose to produce Ethylene! Finally I can connect it with the mature banana</ins>. </html>","tags" : " <ins class="diffchange diffchange-inline">ripenator-mesa-ethylene </ins>"}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"date" : "2013-07-<del class="diffchange diffchange-inline">12</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"author" : "<del class="diffchange diffchange-inline">emil</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"title" : "<del class="diffchange diffchange-inline">Competent cells</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"content" : "<html>I <del class="diffchange diffchange-inline">did some competent cells according </del>to the <del class="diffchange diffchange-inline"><a href=\"https</del>:<del class="diffchange diffchange-inline">//2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </</del>a<del class="diffchange diffchange-inline">></del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"tags" : "<del class="diffchange diffchange-inline">competent cell</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/24&diff=273351&oldid=prevGgirelli at 08:29, 3 October 20132013-10-03T08:29:29Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "date" : "2013-07-<del class="diffchange diffchange-inline">28</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "date" : "2013-07-<ins class="diffchange diffchange-inline">12</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "author" : "<del class="diffchange diffchange-inline">fabio</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "author" : "<ins class="diffchange diffchange-inline">emil</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "title" : " <del class="diffchange diffchange-inline">the blue ligation: the never ending story !</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "title" : "<ins class="diffchange diffchange-inline">Competent cells</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "content" : "<html> <del class="diffchange diffchange-inline"> today </del>I <del class="diffchange diffchange-inline">started with </del>the <del class="diffchange diffchange-inline">fourth ligation and then I transformed for the fourth time</del>.<del class="diffchange diffchange-inline">Meanwhile I minipreppeed inocula from the second ligation, but screening wasn’t successful!!I also inoculated 6 colonies from the third ligation plates( only the plate 1</del>:<del class="diffchange diffchange-inline">1)</del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "content" : "<html>I <ins class="diffchange diffchange-inline">did some competent cells according to </ins>the <ins class="diffchange diffchange-inline"><a href=\"https://2013</ins>.<ins class="diffchange diffchange-inline">igem.org/Team</ins>:<ins class="diffchange diffchange-inline">UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a></ins>.</html>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "tags" : " <del class="diffchange diffchange-inline">YF1_FixJ - FixK2</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "tags" : "<ins class="diffchange diffchange-inline">competent cell</ins>"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/24&diff=110184&oldid=prevGgirelli: moved Team:UNITN-Trento/Notebook/Labposts/123 to Team:UNITN-Trento/Notebook/Labposts/07/242013-09-09T07:26:46Z<p>moved <a href="/Team:UNITN-Trento/Notebook/Labposts/123" class="mw-redirect" title="Team:UNITN-Trento/Notebook/Labposts/123">Team:UNITN-Trento/Notebook/Labposts/123</a> to <a href="/Team:UNITN-Trento/Notebook/Labposts/07/24" title="Team:UNITN-Trento/Notebook/Labposts/07/24">Team:UNITN-Trento/Notebook/Labposts/07/24</a></p>
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<td colspan='1' style="background-color: white; color:black;">Revision as of 07:26, 9 September 2013</td>
</tr></table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/24&diff=78617&oldid=prevGgirelli: Created page with "{ "date" : "2013-07-28", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : "<html> today I started with the fourth ligation and then ..."2013-08-19T13:43:09Z<p>Created page with "{ "date" : "2013-07-28", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : "<html> today I started with the fourth ligation and then ..."</p>
<p><b>New page</b></p><div>{<br />
"date" : "2013-07-28",<br />
"author" : "fabio",<br />
"title" : " the blue ligation: the never ending story !",<br />
"content" : "<html> today I started with the fourth ligation and then I transformed for the fourth time.Meanwhile I minipreppeed inocula from the second ligation, but screening wasn’t successful!!I also inoculated 6 colonies from the third ligation plates( only the plate 1:1).</html>",<br />
"tags" : " YF1_FixJ - FixK2"<br />
}</div>Ggirelli