Team:UNITN-Trento/Notebook/Labposts/07/26

From 2013.igem.org

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{
{
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"date" : "2013-07-27",
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"date" : "2013-07-14",
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"author" : "thomas",
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"author" : "gabriele",
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"title" : "AraCpBAD + EFE + Venus transformation",
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"title" : "Inocula",
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"content" : "<html>Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37&deg;C overnight.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/53/Tn-2013_efe%2Bvenus_plate.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.</html>",
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"content" : "<html>I prepared 3 inocula from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-12-gabriele\">yesterday</a>'s plates:<ul><li>Two inocula from the 1:1 plate.</li><li>One inocula from the 1:2 plate.</li></ul>All the inocula medium contained cloramphenycol.</html>",
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"tags" : "EFE-Venus"
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"tags" : "SAMsynthetase"
}
}

Revision as of 08:30, 3 October 2013

{ "date" : "2013-07-14", "author" : "gabriele", "title" : "Inocula", "content" : "I prepared 3 inocula from yesterday's plates:

  • Two inocula from the 1:1 plate.
  • One inocula from the 1:2 plate.
All the inocula medium contained cloramphenycol.", "tags" : "SAMsynthetase" }