Team:UNITN-Trento/Notebook/Labposts/07/28

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
{
{
-
"date" : "2013-07-30",
+
"date" : "2013-07-15",
"author" : "thomas",
"author" : "thomas",
-
"title" : "Bacillus transformation!!!",
+
"title" : "Ethylene production kinetic assay (third and last trial)",
-
"content" : "<html>In order to test the transformation protocol for <i>B. subtilis</i> that Groeningen team kindly sent to us I prepared a competent cell Media following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation\">this protocol</a>. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following <a href=\"\">this protocol</a>. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/e/ec/Tn-2013_bacillus_transformation_plate_1.jpg\" style =\"width: 450px\"></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Bacillus microscope image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/6/65/Tn-2013_gram_staining_bacillus.jpg\" style =\"width: 450px\"></center></html>}}<html> As you can see from the pictures, seems that <i>Bacillus</i> was successful transformed!!!!</html>",
+
"content" : "<html>Since I was not satisfied with the curve obtained during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-08-thomas\">last experiment</a>, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/00/Tn-2013_kinetic_EFE_plot-2.png\" style =\"width: 450px\"></center></html>}}<html>Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.</html>",
-
"tags" : "b. subtilis-pspac"
+
"tags" : "EFE"
}
}

Revision as of 08:32, 3 October 2013

{ "date" : "2013-07-15", "author" : "thomas", "title" : "Ethylene production kinetic assay (third and last trial)", "content" : "Since I was not satisfied with the curve obtained during the last experiment, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.

Plot
Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.", "tags" : "EFE" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/07/28"