Team:UNITN-Trento/Notebook/Labposts/07/33

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(Difference between revisions)

Revision as of 08:33, 3 October 2013

{ "date" : "2013-07-18", "author" : "emil", "title" : "The incompetent Bacillus subtilis saga medium episode ", "content" : "I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10β following the competent cell protocol .", "tags" : "Bacillus_subtilis" }

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 {
-	"date" : "2013-07-29",
+	"date" : "2013-07-18",
 	"author" : "emil",
-	"title" : "The incompetent Bacillus saga:the final transformation",
+	"title" : "The incompetent Bacillus subtilis saga medium episode ",
-	"content" : "<html>Finally we have decided to try to transform PXyl+GFP that is an integrative vector for the thr locus.I have digested 1&micro;g PXyl with the enzyme Sca1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> standard protocol for the double digestion </a>and exploiting the specific buffer included in the kit from Biolabs.I have previously grown B. subtilis in the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'>medium from Groeningen </a> o.n.,this morning I diluted the Bacillus(1:100) in the same medium then when it reached the O.D. of 1.1 I added 1&micro;l of DNA and I let grow for two hours.After that I plated on spectinomicin added LB agar(stock solution 100mg/ml).</html>",
+	"content" : "<html>I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10&beta; following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a>.</html>",
-	"tags" : "B.subtilis-PXyl"
+	"tags" : "Bacillus_subtilis"
 }