Team:UNITN-Trento/Notebook/Labposts/07/33

From 2013.igem.org

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{"date" : "2013-07-15","author" : "thomas","title" : "Ethylene production kinetic assay (third and last trial)","content" : "<html>Since I was not satisfied with the curve obtained during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-08-thomas\">last experiment</a>, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/00/Tn-2013_kinetic_EFE_plot-2.png\" style =\"width: 450px\"></center></html>}}<html>Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.</html>","tags" : "EFE"}
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"date" : "2013-07-18",
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"author" : "emil",
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"title" : "The incompetent Bacillus subtilis saga medium episode ",
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"content" : "<html>I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10&beta; following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a>.</html>",
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"tags" : "Bacillus_subtilis"
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}
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Latest revision as of 10:40, 3 October 2013

{"date" : "2013-07-15","author" : "thomas","title" : "Ethylene production kinetic assay (third and last trial)","content" : "Since I was not satisfied with the curve obtained during the last experiment, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.

Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.","tags" : "EFE"}