Team:UNITN-Trento/Notebook/Labposts/07/40

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Revision as of 08:35, 3 October 2013

{ "date" : "2013-07-20", "author" : "fabio-bruno", "title" : " blue and red light sensors!!", "content" : " this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula.
Second set yields are:
006a : 289
006b : 409.3
016° : 188.8
016b :266.6
030a : 388.6
030b : 270
082a : 433.0
082b : 568
Third set yiealds are:
006: 321 ng/ul
016: 440.9 ng/ul
R0082: 441,5 ng/ul
030: 199,9 ng/ul
These ones haven’t been screened so far.
Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.", "tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-02",
+	"date" : "2013-07-20",
-	"author" : "caterina-michele",
+	"author" : "fabio-bruno",
-	"title" : "Incredible: E.coli could growth!",
+	"title" : " blue and red light sensors!!",
-	"content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ",
+	"content" : "<html> this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula. <br>Second set yields are: <br>006a : 289 <br>006b : 409.3<br>016° : 188.8<br>016b :266.6<br>030a : 388.6<br>030b : 270<br>082a : 433.0<br>082b : 568<br>Third set yiealds are: <br>006: 321 ng/ul<br>016: 440.9 ng/ul<br>R0082: 441,5 ng/ul<br>030: 199,9 ng/ul<br>These ones haven’t been screened so far. <br>Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.</html>",
-	"tags" : "PchBA-BMST1-araC-pBAD"
+	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
 }