From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-07-08", | + | "date" : "2013-07-22", |
| - | "author" : "emil", | + | "author" : "thomas", |
| - | "title" : " Purification of Inocula (03/7)", | + | "title" : "Inocula of AraC-pBAD + EFE + Venus", |
| - | "content" : "<html> I verifyed the status of the last inocula(05/7) that resulted a bit strange.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Inocula|<html><img src=\"\" width=\"450px\" /></html>}}<html>Unfortunately The day before were inoculate uncorrect plates(03/7) so I have purified them following the <a href=\"https://2013.igem.org/ Team:UNITN-Trento/ Protocols#miniprep\"> purification protocol </a> these are the results of the quantification. </html>{{:Team:UNITN-Trento/ Templates/Styles/Spoiler|Quantification|<html><center><table class=\"tn- sp- table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 1</td><td>438.8 ng/µl</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 2</td><td>400.7 ng/µl</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) 3</td><td>343.6 ng/µl(the only succesful)</td></tr></table></center></html>}}<html>Afterwards I screened 800 ng of the samples following the <a href=\"https://2013 .igem.org/Team:UNITN- Trento/Protocols#Digestion\">screening protocol</a> with EcoR1 HF and Pst1, these are the results of the gel:</html>{{:Team:UNITN- Trento/Templates/Styles/Spoiler|Gel order|<html><center><table class=\"tn- sp- table\"> <tr><th>Sample</ th><th>Well</th></tr><tr><td>Ladder 1kb Fermentas</td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) a </td><td>3</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) b</td><td>4</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) </td><td>5 </td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><img src=\"\" width=\"450px\" /></html>}}<html>As we can see only the third(024) sample shows the insert at the right size of 1000 bp(the lower band), then I did the inocula of the plates of 5/07(1:1 ,1:2,1:3) and of the old plate that gives right results to amplify 024(1: 1).</html>", | + | "content" : "<html> This is the part four of <a href=\"https://2013.igem.org/ wiki/ index. php?title=Team:UNITN-Trento/ Notebook#tn- post-2013- 07- 18- thomas- michele\"> this experiment</a> . I proceeded preparing 5 inocula from the 1:1 and 1: 4 ligation product Plates. The inocula were grown overnight at 37°C in thermoshaker. </html>", |
| - | "tags" : "K823024-K823026-E0840" | + | "tags" : "EFE-Venus" |
| | } | | } |
Revision as of 08:36, 3 October 2013
{
"date" : "2013-07-22",
"author" : "thomas",
"title" : "Inocula of AraC-pBAD + EFE + Venus",
"content" : "This is the part four of this experiment. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37°C in thermoshaker. ",
"tags" : "EFE-Venus"
}
"
