Team:UNITN-Trento/Notebook/Labposts/07/46

From 2013.igem.org

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{"date" : "2013-07-21","author" : "thomas","title" : "Ligation of AraC-pBAD + EFE and Venus","content" : "<html>This is the part three of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. The digestion products were ligated together following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">this protocol</a>. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.</html>","tags" : "EFE-Venus"}
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"date" : "2013-07-09",
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"author" : "Bruno",
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"title" : "Ripenator v1.0",
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"content" : "<html>I set up a system to ripening or block ripening a set of green (immature) bananas. <br>The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected. <br>In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.</html>",
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"tags" : "Ripenator-Mesa-Ethylene"
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}
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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-21","author" : "thomas","title" : "Ligation of AraC-pBAD + EFE and Venus","content" : "This is the part three of this experiment. The digestion products were ligated together following this protocol. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.","tags" : "EFE-Venus"}