Team:UNITN-Trento/Notebook/Labposts/07/46

From 2013.igem.org

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{
{
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"date" : "2013-07-09",
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"date" : "2013-07-22",
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"author" : "Bruno",
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"author" : "emil",
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"title" : "Ripenator v1.0",
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"title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ",
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"content" : "<html>I set up a system to ripening or block ripening a set of green (immature) bananas. <br>The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected. <br>In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.</html>",
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"content" : "<html>Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.</html>",
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"tags" : "Ripenator-Mesa-Ethylene"
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"tags" : "Bacillus subtilis"
}
}

Revision as of 08:37, 3 October 2013

{ "date" : "2013-07-22", "author" : "emil", "title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ", "content" : "Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.", "tags" : "Bacillus subtilis" }