Team:UNITN-Trento/Notebook/Labposts/07/46

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(Difference between revisions)

Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-21","author" : "thomas","title" : "Ligation of AraC-pBAD + EFE and Venus","content" : "This is the part three of this experiment. The digestion products were ligated together following this protocol. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.","tags" : "EFE-Venus"}

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+{"date" : "2013-07-21","author" : "thomas","title" : "Ligation of AraC-pBAD + EFE and Venus","content" : "<html>This is the part three of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. The digestion products were ligated together following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">this protocol</a>. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.</html>","tags" : "EFE-Venus"}
-	"date" : "2013-07-22",
-	"author" : "emil",
-	"title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ",
-	"content" : "<html>Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.</html>",
-	"tags" : "Bacillus subtilis"
-}