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{"date" : "2013-07-21","author" : "thomas","title" : "Ligation of AraC-pBAD + EFE and Venus","content" : "This is the part three of this experiment. The digestion products were ligated together following this protocol. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.","tags" : "EFE-Venus"}