Team:UNITN-Trento/Notebook/Labposts/07/50

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(Created page with "{ "date" : "2013-07-03", "author" : "gabriele", "title" : "An easy day...", "content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/in...")
 
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{"date" : "2013-07-22","author" : "thomas","title" : "Inocula of AraC-pBAD + EFE + Venus","content" : "<html>This is the part four of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37&deg;C in thermoshaker. </html>","tags" : "EFE-Venus"}
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"date" : "2013-07-03",
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"author" : "gabriele",
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"title" : "An easy day...",
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"content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-gabriele\">yesterday</a>:<br/><br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th></tr><tr><td>Ctrl</td><td>4</td></tr><tr><td>0.5:1</td><td>2</td></tr><tr><td>0.5:2</td><td>0</td></tr></table></center><br/><hr><br/>Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th colspan=\"4\">Loading scheme</th></tr><tr><td>1kb ladder</td><td>G1</td><td>G2</td><td>G3</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/d/d3/Tn-20130703-GGCM_linearizationpSB1C3_FAILED.jpg\" alt=\"Gel\" width=\"450px\"></center></html>}}<html></html>",
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"tags" : "SAMsynthetase-pSB1C3"
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}
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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-22","author" : "thomas","title" : "Inocula of AraC-pBAD + EFE + Venus","content" : "This is the part four of this experiment. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37°C in thermoshaker. ","tags" : "EFE-Venus"}