Team:UNITN-Trento/Notebook/Labposts/07/50

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Revision as of 08:38, 3 October 2013

{ "date" : "2013-07-23", "author" : "bruno", "title" : "Blue sensor", "content" : "I made mini prep from inoculum overnight. I oubtained the following yields:

Quantification Results

Sample	Concentration
K952003-1	1019,1 ng/ul
K952003-2	1715,7 ng/ul
K952003-3	180,0 ng/ul
K952003-4	236,4 ng/ul

from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.", "tags" : "BBa_K952003" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-03",
+	"date" : "2013-07-23",
-	"author" : "gabriele",
+	"author" : "bruno",
-	"title" : "An easy day...",
+	"title" : "Blue sensor",
-	"content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-gabriele\">yesterday</a>:<br/><br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th></tr><tr><td>Ctrl</td><td>4</td></tr><tr><td>0.5:1</td><td>2</td></tr><tr><td>0.5:2</td><td>0</td></tr></table></center><br/><hr><br/>Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th colspan=\"4\">Loading scheme</th></tr><tr><td>1kb ladder</td><td>G1</td><td>G2</td><td>G3</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/d/d3/Tn-20130703-GGCM_linearizationpSB1C3_FAILED.jpg\" alt=\"Gel\" width=\"450px\"></center></html>}}<html></html>",
+	"content" : "<html>I made mini prep from inoculum overnight. I oubtained the following yields:<br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>K952003-1 </td><td>1019,1 ng/ul</td></tr><tr><td>K952003-2</td><td>1715,7 ng/ul</td></tr><tr><td>K952003-3 </td><td>180,0  ng/ul</td></tr><tr><td>K952003-4</td><td>236,4 ng/ul</td></tr></TABLE></center></html>}}<html><br/>from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.</html>",
-	"tags" : "SAMsynthetase-pSB1C3"
+	"tags" : "BBa_K952003"
 }