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Team:UNITN-Trento/Notebook/Labposts/07/56
From 2013.igem.org
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{ | { | ||
| - | "date" : "2013-07- | + | "date" : "2013-07-27", |
| - | "author" : " | + | "author" : "fabio", |
| - | "title" : " | + | "title" : " the blue ligation: the never ending story !", |
| - | "content" : "<html>I | + | "content" : "<html> I first added sap to the o/n digestion( third), then purified and quantified : yield, 32,3 ng/ul.Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b.In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng/ul for 016 and 11,8ng/ul for oo6. Tomorrow I will continue with the ligation number 4.</html>", |
| - | "tags" : " | + | "tags" : " YF1_FixJ - FixK2" |
} | } | ||
Revision as of 08:44, 3 October 2013
{ "date" : "2013-07-27", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : " I first added sap to the o/n digestion( third), then purified and quantified : yield, 32,3 ng/ul.Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b.In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng/ul for 016 and 11,8ng/ul for oo6. Tomorrow I will continue with the ligation number 4.", "tags" : " YF1_FixJ - FixK2" }
