http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/56&feed=atom&action=historyTeam:UNITN-Trento/Notebook/Labposts/07/56 - Revision history2024-03-28T13:54:22ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/56&diff=274529&oldid=prevGgirelli at 10:45, 3 October 20132013-10-03T10:45:46Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{"date" : "2013-07-<ins class="diffchange diffchange-inline">24</ins>","author" : "fabio<ins class="diffchange diffchange-inline">-bruno</ins>","title" : " the blue ligation: the never ending story !","content" : "<html><ins class="diffchange diffchange-inline">Today </ins>I <ins class="diffchange diffchange-inline">inoculated 6 colonies of </ins>the <ins class="diffchange diffchange-inline">plated ligation </ins>( 3 <ins class="diffchange diffchange-inline">from </ins>the <ins class="diffchange diffchange-inline">1:1 plate </ins>and <ins class="diffchange diffchange-inline">2 from </ins>the <ins class="diffchange diffchange-inline">1:3 plate) <br></ins>I <ins class="diffchange diffchange-inline">also </ins>made <ins class="diffchange diffchange-inline">the Onetaq Pcr for all the light parts ( blue device,the b sample </ins>from the <ins class="diffchange diffchange-inline">first set; red device, from the third set; </ins>and <ins class="diffchange diffchange-inline">red promoter, from </ins>the <ins class="diffchange diffchange-inline">third set). <br>After that </ins>I <ins class="diffchange diffchange-inline">screened them all </ins>and <ins class="diffchange diffchange-inline">found out that </ins>only the <ins class="diffchange diffchange-inline">blue part was confirmed</ins>: <ins class="diffchange diffchange-inline">up to now we only have the blue parts confirmed!! Red parts </ins>, <ins class="diffchange diffchange-inline">it seems to me that you’re in hot waters !! <br><</ins>/<ins class="diffchange diffchange-inline">html>{{:Team:UNITN-Trento</ins>/<ins class="diffchange diffchange-inline">Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static</ins>.<ins class="diffchange diffchange-inline">igem</ins>.<ins class="diffchange diffchange-inline">org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></ins></html>","tags" : " YF1_FixJ - FixK2<ins class="diffchange diffchange-inline">- ho1_PcyA_cph8-OmpR</ins>"}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"date" : "2013-07-<del class="diffchange diffchange-inline">27</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"author" : "fabio",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"title" : " the blue ligation: the never ending story !",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"content" : "<html> I <del class="diffchange diffchange-inline">first added sap to </del>the <del class="diffchange diffchange-inline">o/n digestion</del>( <del class="diffchange diffchange-inline">third), then purified and quantified : yield, 32,</del>3 <del class="diffchange diffchange-inline">ng/ul.Then again I ligated 006 to 016 folowing </del>the <del class="diffchange diffchange-inline">protocol </del>and <del class="diffchange diffchange-inline">plated 10 ul in 200 ul of neb10b.In </del>the <del class="diffchange diffchange-inline">afternoon </del>I made <del class="diffchange diffchange-inline">6 inocula </del>from the <del class="diffchange diffchange-inline">second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid </del>and <del class="diffchange diffchange-inline">016 as </del>the <del class="diffchange diffchange-inline">insert!! </del>I <del class="diffchange diffchange-inline">need to digest the two part with different enzymes E </del>and <del class="diffchange diffchange-inline">X for 006 and E and S for 016! This time I digested 2400 ng for </del>only <del class="diffchange diffchange-inline">5 ours, not all </del>the <del class="diffchange diffchange-inline">night</del>: <del class="diffchange diffchange-inline">yields are</del>, <del class="diffchange diffchange-inline">25 ng</del>/<del class="diffchange diffchange-inline">ul for 016 and 11,8ng</del>/<del class="diffchange diffchange-inline">ul for oo6</del>. <del class="diffchange diffchange-inline">Tomorrow I will continue with the ligation number 4</del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"tags" : " YF1_FixJ - FixK2"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/56&diff=273444&oldid=prevGgirelli at 08:44, 3 October 20132013-10-03T08:44:05Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "date" : "2013-07-<del class="diffchange diffchange-inline">12</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "date" : "2013-07-<ins class="diffchange diffchange-inline">27</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "author" : "<del class="diffchange diffchange-inline">emil</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "author" : "<ins class="diffchange diffchange-inline">fabio</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "title" : "<del class="diffchange diffchange-inline">Competent cells</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "title" : " <ins class="diffchange diffchange-inline">the blue ligation: the never ending story !</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "content" : "<html>I <del class="diffchange diffchange-inline">did some competent cells according </del>to the <del class="diffchange diffchange-inline"><a href=\"https</del>:/<del class="diffchange diffchange-inline">/2013</del>.<del class="diffchange diffchange-inline">igem</del>.<del class="diffchange diffchange-inline">org/Team</del>:<del class="diffchange diffchange-inline">UNITN-Trento</del>/<del class="diffchange diffchange-inline">Protocols#Competent-cells-prep\"> competent cell protocol <</del>/<del class="diffchange diffchange-inline">a></del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "content" : "<html> I <ins class="diffchange diffchange-inline">first added sap </ins>to the <ins class="diffchange diffchange-inline">o/n digestion( third), then purified and quantified </ins>: <ins class="diffchange diffchange-inline">yield, 32,3 ng</ins>/<ins class="diffchange diffchange-inline">ul</ins>.<ins class="diffchange diffchange-inline">Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b</ins>.<ins class="diffchange diffchange-inline">In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy</ins>: <ins class="diffchange diffchange-inline">using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng</ins>/<ins class="diffchange diffchange-inline">ul for 016 and 11,8ng</ins>/<ins class="diffchange diffchange-inline">ul for oo6. Tomorrow I will continue with the ligation number 4</ins>.</html>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "tags" : "<del class="diffchange diffchange-inline">competent cell</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "tags" : " <ins class="diffchange diffchange-inline">YF1_FixJ - FixK2</ins>"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/56&diff=110471&oldid=prevGgirelli: moved Team:UNITN-Trento/Notebook/Labposts/89 to Team:UNITN-Trento/Notebook/Labposts/07/562013-09-09T07:54:08Z<p>moved <a href="/Team:UNITN-Trento/Notebook/Labposts/89" class="mw-redirect" title="Team:UNITN-Trento/Notebook/Labposts/89">Team:UNITN-Trento/Notebook/Labposts/89</a> to <a href="/Team:UNITN-Trento/Notebook/Labposts/07/56" title="Team:UNITN-Trento/Notebook/Labposts/07/56">Team:UNITN-Trento/Notebook/Labposts/07/56</a></p>
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<td colspan='1' style="background-color: white; color:black;">Revision as of 07:54, 9 September 2013</td>
</tr></table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/56&diff=78564&oldid=prevGgirelli: Created page with "{ "date" : "2013-07-12", "author" : "emil", "title" : "Competent cells", "content" : "<html>I did some competent cells according to the <a href=\"https://2013.igem.org/Team:UN..."2013-08-19T13:35:01Z<p>Created page with "{ "date" : "2013-07-12", "author" : "emil", "title" : "Competent cells", "content" : "<html>I did some competent cells according to the <a href=\"https://2013.igem.org/Team:UN..."</p>
<p><b>New page</b></p><div>{<br />
"date" : "2013-07-12",<br />
"author" : "emil",<br />
"title" : "Competent cells",<br />
"content" : "<html>I did some competent cells according to the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a>.</html>",<br />
"tags" : "competent cell"<br />
}</div>Ggirelli