http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/61&feed=atom&action=historyTeam:UNITN-Trento/Notebook/Labposts/07/61 - Revision history2024-03-29T07:38:29ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/61&diff=274544&oldid=prevGgirelli at 10:46, 3 October 20132013-10-03T10:46:45Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{"date" : "2013-07-<ins class="diffchange diffchange-inline">26</ins>","author" : "<ins class="diffchange diffchange-inline">viola</ins>","title" : "<ins class="diffchange diffchange-inline"><html>I'm near </ins>the <ins class="diffchange diffchange-inline">solution...</html></ins>","content" : "<html><ins class="diffchange diffchange-inline">Poor bacillus!!! Fried in a super basic pH...the media that </ins>we <ins class="diffchange diffchange-inline">used were too basic! so tonight i tried </ins>to <ins class="diffchange diffchange-inline">bring </ins>to <ins class="diffchange diffchange-inline">pH 7 </ins>the <ins class="diffchange diffchange-inline">Groningen</ins>'<ins class="diffchange diffchange-inline">s media and tomorrow wi we</ins>'<ins class="diffchange diffchange-inline">ll see </ins>the <ins class="diffchange diffchange-inline">truth</ins>... <ins class="diffchange diffchange-inline">meanwhile i prepared </ins>the <ins class="diffchange diffchange-inline">samples for </ins>the <ins class="diffchange diffchange-inline">second sequencing of pspac and pxyl vectors with EFE</ins>... <ins class="diffchange diffchange-inline">i hope </ins>that <ins class="diffchange diffchange-inline">this time there will be something good!!!! </ins></html>","tags" : "<ins class="diffchange diffchange-inline">Bacillus</ins>"}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"date" : "2013-07-<del class="diffchange diffchange-inline">29</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"author" : "<del class="diffchange diffchange-inline">emil</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"title" : "<del class="diffchange diffchange-inline">The incompetent Bacillus saga:</del>the <del class="diffchange diffchange-inline">final transformation</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"content" : "<html><del class="diffchange diffchange-inline">Finally </del>we <del class="diffchange diffchange-inline">have decided </del>to <del class="diffchange diffchange-inline">try </del>to <del class="diffchange diffchange-inline">transform PXyl+GFP that is an integrative vector for </del>the <del class="diffchange diffchange-inline">thr locus.I have digested 1&micro;g PXyl with the enzyme Sca1 following the <a href=</del>'<del class="diffchange diffchange-inline">https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion</del>'<del class="diffchange diffchange-inline">> standard protocol for </del>the <del class="diffchange diffchange-inline">double digestion </a>and exploiting the specific buffer included in the kit from Biolabs</del>.<del class="diffchange diffchange-inline">I have previously grown B</del>. <del class="diffchange diffchange-inline">subtilis in the <a href='https://2013</del>.<del class="diffchange diffchange-inline">igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'>medium from Groeningen </a> o.n.,this morning I diluted </del>the <del class="diffchange diffchange-inline">Bacillus(1:100) in </del>the <del class="diffchange diffchange-inline">same medium then when it reached the O</del>.<del class="diffchange diffchange-inline">D</del>. <del class="diffchange diffchange-inline">of 1</del>.<del class="diffchange diffchange-inline">1 I added 1&micro;l of DNA and I let grow for two hours.After </del>that <del class="diffchange diffchange-inline">I plated on spectinomicin added LB agar(stock solution 100mg/ml).</del></html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>"tags" : "<del class="diffchange diffchange-inline">B.subtilis-PXyl</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/61&diff=273452&oldid=prevGgirelli at 08:45, 3 October 20132013-10-03T08:45:39Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:45, 3 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "date" : "2013-07-<del class="diffchange diffchange-inline">16</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "date" : "2013-07-<ins class="diffchange diffchange-inline">29</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "author" : "<del class="diffchange diffchange-inline">gabriele</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "author" : "<ins class="diffchange diffchange-inline">emil</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "title" : "<del class="diffchange diffchange-inline">A short day</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "title" : "<ins class="diffchange diffchange-inline">The incompetent Bacillus saga:the final transformation</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "content" : "<html>I <del class="diffchange diffchange-inline">added </del>1&micro;<del class="diffchange diffchange-inline">l of DpnI to G1_EX</del>-<del class="diffchange diffchange-inline">SAMsynth-SP O</del>/<del class="diffchange diffchange-inline">N </del>digestion and <del class="diffchange diffchange-inline">1&micro;l of SAP to G2A_linear</del>-<del class="diffchange diffchange-inline">pSB1C3 O</del>/<del class="diffchange diffchange-inline">N digestion</del>, <del class="diffchange diffchange-inline">and then incubated both samples at 37&deg;C for </del>1.<del class="diffchange diffchange-inline">5h</del>. <del class="diffchange diffchange-inline">Finally, </del>I <del class="diffchange diffchange-inline">stocked both samples at -20</del>&<del class="diffchange diffchange-inline">deg</del>;<del class="diffchange diffchange-inline">C because </del>I <del class="diffchange diffchange-inline">would like to ligate them together with the short digestion </del>that I <del class="diffchange diffchange-inline">am going to perform tomorrow</del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "content" : "<html><ins class="diffchange diffchange-inline">Finally we have decided to try to transform PXyl+GFP that is an integrative vector for the thr locus.</ins>I <ins class="diffchange diffchange-inline">have digested </ins>1&micro;<ins class="diffchange diffchange-inline">g PXyl with the enzyme Sca1 following the <a href='https://2013.igem.org/Team:UNITN</ins>-<ins class="diffchange diffchange-inline">Trento</ins>/<ins class="diffchange diffchange-inline">Protocols#Digestion'> standard protocol for the double </ins>digestion <ins class="diffchange diffchange-inline"></a></ins>and <ins class="diffchange diffchange-inline">exploiting the specific buffer included in the kit from Biolabs.I have previously grown B. subtilis in the <a href='https://2013.igem.org/Team:UNITN</ins>-<ins class="diffchange diffchange-inline">Trento</ins>/<ins class="diffchange diffchange-inline">Protocols#subtilis-transformation'>medium from Groeningen </a> o.n.</ins>,<ins class="diffchange diffchange-inline">this morning I diluted the Bacillus(</ins>1<ins class="diffchange diffchange-inline">:100) in the same medium then when it reached the O</ins>.<ins class="diffchange diffchange-inline">D</ins>. <ins class="diffchange diffchange-inline">of 1.1 </ins>I <ins class="diffchange diffchange-inline">added 1</ins>&<ins class="diffchange diffchange-inline">micro</ins>;<ins class="diffchange diffchange-inline">l of DNA and </ins>I <ins class="diffchange diffchange-inline">let grow for two hours.After </ins>that I <ins class="diffchange diffchange-inline">plated on spectinomicin added LB agar(stock solution 100mg/ml)</ins>.</html>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "tags" : "<del class="diffchange diffchange-inline">SAMsynthetase</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "tags" : "<ins class="diffchange diffchange-inline">B.subtilis-PXyl</ins>"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/61&diff=110489&oldid=prevGgirelli: moved Team:UNITN-Trento/Notebook/Labposts/94 to Team:UNITN-Trento/Notebook/Labposts/07/612013-09-09T07:56:32Z<p>moved <a href="/Team:UNITN-Trento/Notebook/Labposts/94" class="mw-redirect" title="Team:UNITN-Trento/Notebook/Labposts/94">Team:UNITN-Trento/Notebook/Labposts/94</a> to <a href="/Team:UNITN-Trento/Notebook/Labposts/07/61" title="Team:UNITN-Trento/Notebook/Labposts/07/61">Team:UNITN-Trento/Notebook/Labposts/07/61</a></p>
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</tr></table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/07/61&diff=78569&oldid=prevGgirelli: Created page with "{ "date" : "2013-07-16", "author" : "gabriele", "title" : "A short day", "content" : "<html>I added 1µl of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1µl of SAP ..."2013-08-19T13:35:58Z<p>Created page with "{ "date" : "2013-07-16", "author" : "gabriele", "title" : "A short day", "content" : "<html>I added 1µl of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1µl of SAP ..."</p>
<p><b>New page</b></p><div>{<br />
"date" : "2013-07-16",<br />
"author" : "gabriele",<br />
"title" : "A short day",<br />
"content" : "<html>I added 1&micro;l of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1&micro;l of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37&deg;C for 1.5h. Finally, I stocked both samples at -20&deg;C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.</html>",<br />
"tags" : "SAMsynthetase"<br />
}</div>Ggirelli