From 2013.igem.org
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| - | {
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| - | "date":"2013-07-06",
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| - | "author":"viola",
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| - | "title":"<html>cloning of EFE in pSpac and pXyl vectors</html>",
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| - | "content":"<html> Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1| <html> <center> <table> <tr> <th>LIGATION</th> <th>EFE+B0015 in pSPAC</th> <th>EFE+B0015 in pXYL</th> </tr> <tr> <th>PLASMID CONC.</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>PLASMID LENGHT</th> <td>7082 BP</td> <td>3594 BP</td> </tr> <tr> <th>INSERT CONCENTRATION</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>INSERT LENGHT</th> <td>1251 BP</td> <td>1251 BP</td> </tr> <tr> <th>PLASMID USED</th> <td>50 ng/µl</td> <td>50 ng/µl</td> </tr> <tr> <th>REACTION VOLUME</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>BUFFER</th> <td>10 µl</td> <td>10 µl</td> </tr> </table> </center> </html>}} <html> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PSPAC| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PSPAC</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.44</td> <td>0.88</td> <td>1.76</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>14.06</td> <td>13.62</td> <td>12.74</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PXYL| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PXYL</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.87</td> <td>1.74</td> <td>3.48</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>13.63</td> <td>12.76</td> <td>11.02</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} <html> </html>}} <html> </html>}} </html>",
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| - | "tags":"BACILLUS"
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| - | }
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Latest revision as of 10:50, 3 October 2013
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