Team:UNITN-Trento/Notebook/Labposts/08/08

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(Difference between revisions)
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{
{
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"date" : "2013-08-06",
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"date" : "2013-08-03",
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"author" : "bruno-viola-caterina-gabriele-thomas",
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"author" : "gabriele",
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"title" : "Surrender now or prepare to fight, Meowth! That's right.",
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"title" : "Wintergreen with pLac-SAMsynthetase: Ep. II",
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"content" : "<html>Today we made 5 different competent cells following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a>:<br/>- NEB10&beta;<br/>- TB1<br/>- TOP10<br/>- JM109<br/>- BL21<br/><br/>Then we trasformed in these cells R0010-K592009 in psB1C3 and K1065300. <br/><br/><br/><br/><br/><br/><br/><br/></html>",
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"content" : "<html>I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/&micro;l) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!! </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>13A</td></tr><tr><td>13B</td></tr><tr><td>13C</td></tr><tr><td><i>Empty</i></td></tr><tr><td>14A</td></tr><tr><td>14B</td></tr><tr><td>14C</td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/f/f7/Tn-20130803_SAMinWGREEN_check_2.jpg\" /></html>}}<html>Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>1kb ladder</td></tr><tr><td>13A</td></tr><tr><td>Ctrl</td></tr><tr><td><i>14B</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/7/76/Tn-20130803_SAMinWGREEN_check_3.jpg\" /></html>}}<html></html>",
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"tags" : "cells competent"
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"tags" : "Wintergreen-SAMsynthetase-pLac"
}
}

Revision as of 09:03, 3 October 2013

{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/µl) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!!

Screening Gel
Loading scheme
13A
13B
13C
Empty
14A
14B
14C
1kb ladder
Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!
Screening Gel
Loading scheme
1kb ladder
13A
Ctrl
14B
1kb ladder
", "tags" : "Wintergreen-SAMsynthetase-pLac" }

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