Team:UNITN-Trento/Notebook/Labposts/08/08

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{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/µl) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!!

Screening Gel
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13A
13B
13C
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14A
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14C
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Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!
Screening Gel
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13A
Ctrl
14B
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", "tags" : "Wintergreen-SAMsynthetase-pLac" }