Team:UNITN-Trento/Notebook/Labposts/08/16

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Revision as of 09:05, 3 October 2013

{ "date" : "2013-08-07", "author" : "viola", "title" : "cloning cloning cloning!", "content" : "The plates that i did yesterday are quite perfect! The ctrl plate is quite empty and there are many colonies on the other samples (1:1,1:2,1:3,1:4). This evening i will do some inocula. Today I'm making also the screening of yesterday's inocula from that bad plates and from the gel we can see that the ligation worked only in the third sample:

Gel image

", "tags" : "s04617-EFE" }

@@ Line 1: / Line 1: @@
 {
 	"date" : "2013-08-07",
-	"author" : "emil",
+	"author" : "viola",
-	"title" : "Test for integration in B. subtilis ",
+	"title" : "cloning cloning cloning!",
-	"content" : "<html>In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MNGE medium|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>10xMN-Medium</td><td>3,68ml</td></tr><tr><td>Sterile water</td><td>33,12ml</td></tr><tr><td>Glucose (20%)</td><td>4ml</td></tr><tr><td>K-Glutamat (40%)</td><td>200&micro;l</td></tr><tr><td>Fe[III]-ammonium-citrate (2,2 mg/ml)</td><td>200&micro;l</td></tr><tr><td>Tryptophan (5 mg/ml)</td><td>400&micro;l</td></tr><tr><td>MgSO4 (1M)</td><td>120&micro;l</td></tr><tr><td>threonine (5 mg/ml)(in this case absent)</td><td>400&micro;l</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MN medium 10x 50 ml|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>K2HPO4 (x3 H2O)</td><td>6,8 g</td></tr><tr><td>KH2PO4</td><td>3 g</td></tr><tr><td> Na-citrate (x 2 H2O) </td><td>0.5 g</td></tr></table>    </center></html>}}",
+	"content" : "<html>The plates that i did <a href=\"https://2013.igem.org/Team:UNITN-Trento/Notebook?title=Team:UNITN-Trento/Notebook&action=edit&section=138\">yesterday</a> are quite perfect! The ctrl plate is quite empty and there are many colonies on the other samples (1:1,1:2,1:3,1:4). This evening i will do some inocula. Today I'm making also the screening of yesterday's inocula from that bad plates and from the gel we can see that the ligation worked only in the third sample:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/f/f6/GEL1.png\" width=\"450px\" /></center></html>}}<html> </html>",
-	"tags" : "B.subtilis-PXyl"
+	"tags" : "s04617-EFE"
 }