Team:UNITN-Trento/Notebook/Labposts/08/16

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{
{
"date" : "2013-08-07",
"date" : "2013-08-07",
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"author" : "emil",
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"author" : "gabriele-emil",
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"title" : "Test for integration in B. subtilis ",
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"title" : "M9 Minimal Medium",
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"content" : "<html>In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MNGE medium|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>10xMN-Medium</td><td>3,68ml</td></tr><tr><td>Sterile water</td><td>33,12ml</td></tr><tr><td>Glucose (20%)</td><td>4ml</td></tr><tr><td>K-Glutamat (40%)</td><td>200&micro;l</td></tr><tr><td>Fe[III]-ammonium-citrate (2,2 mg/ml)</td><td>200&micro;l</td></tr><tr><td>Tryptophan (5 mg/ml)</td><td>400&micro;l</td></tr><tr><td>MgSO4 (1M)</td><td>120&micro;l</td></tr><tr><td>threonine (5 mg/ml)(in this case absent)</td><td>400&micro;l</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|MN medium 10x 50 ml|<html><center><table><tr><th>Compound</th><th>Amount</th></tr><tr><td>K2HPO4 (x3 H2O)</td><td>6,8 g</td></tr><tr><td>KH2PO4</td><td>3 g</td></tr><tr><td> Na-citrate (x 2 H2O) </td><td>0.5 g</td></tr></table>    </center></html>}}",
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"content" : "<html>Simply (it is not very easy but we succesed) prepared the M9 minimal medium!</html>",
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"tags" : "B.subtilis-PXyl"
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"tags" : "M9"
}
}

Latest revision as of 09:05, 3 October 2013

{ "date" : "2013-08-07", "author" : "gabriele-emil", "title" : "M9 Minimal Medium", "content" : "Simply (it is not very easy but we succesed) prepared the M9 minimal medium!", "tags" : "M9" }