From 2013.igem.org
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| { | | { |
- | "date" : "2013-08-12", | + | "date" : "2013-08-08", |
- | "author" : "thomas-viola-michele", | + | "author" : "caterina-bruno", |
- | "title" : "Minipreps and screening", | + | "title" : "ligation of our parts", |
- | "content" : "<html>Starting from the o/n cultures prepared <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-11-thomas-viola\">yesterday</a>, we performed a miniprep purification, obtaining these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><table><tr><th>Sample</th><th>Concentration </th></tr><tr><td>S04617 + EFE ligation 1:2 sample 1</td><td>459 ng/µl</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 2</td><td>603.2 ng/µl</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 3</td><td>294.2 ng/µl</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 1</td><td>434.4 ng/µl</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 2</td><td>346.3 ng/µl</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 1</td><td> 351.4 ng/µl</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 2</td><td>293.7 ng/µl</td></tr></table></center></html>}}<html> We proceeded then performing a screening digestion followed by an electrophoresis analysis. Kapa universal ladder was adopted.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/02/Tn2013_GEL_aracpbad-EFE_S04617%2BEFE.jpg\"></center></html>}}<html> As you can see from the image, AraCpBAD + EFE (BBa_K1065002) part is confirmed for all the 3 samples (lane 1 to 3, 2426 bp). Differently, BBa_S04617 + EFE (BBa_K1265002) is not confirmed (lane 5 to 7, 2460 bp expected) since the band is at an heigh of about 1400 bp. Probably the destination vector BBa_S04617 was closed itself without incorporating EFE.</html>", | + | "content" : "<html>Today we performed a digestion of J23100+K592016 with EcoR I and Spe I, and K592020 in pSB1C3 with EcoR I and XbaI to obtain the complete biobrick...waiting tomorrow for the results.</html>", |
- | "tags" : "pFixK-cI-pLambda-EFE-AraCpBAD" | + | "tags" : "K592016-K592020" |
| } | | } |
Latest revision as of 09:08, 3 October 2013
{
"date" : "2013-08-08",
"author" : "caterina-bruno",
"title" : "ligation of our parts",
"content" : "Today we performed a digestion of J23100+K592016 with EcoR I and Spe I, and K592020 in pSB1C3 with EcoR I and XbaI to obtain the complete biobrick...waiting tomorrow for the results.",
"tags" : "K592016-K592020"
}