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Team:UNITN-Trento/Notebook/Labposts/08/21 - Revision history
2024-03-28T09:25:33Z
Revision history for this page on the wiki
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Ggirelli at 09:08, 3 October 2013
2013-10-03T09:08:51Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:08, 3 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "date" : "2013-08-<del class="diffchange diffchange-inline">12</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "date" : "2013-08-<ins class="diffchange diffchange-inline">08</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "author" : "<del class="diffchange diffchange-inline">thomas</del>-<del class="diffchange diffchange-inline">viola-michele</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "author" : "<ins class="diffchange diffchange-inline">caterina</ins>-<ins class="diffchange diffchange-inline">bruno</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "title" : "<del class="diffchange diffchange-inline">Minipreps and screening</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "title" : "<ins class="diffchange diffchange-inline">ligation of our parts</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "content" : "<html><del class="diffchange diffchange-inline">Starting from the o/n cultures prepared <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-11-thomas-viola\">yesterday</a>, </del>we performed a <del class="diffchange diffchange-inline">miniprep purification, obtaining these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><table><tr><th>Sample</th><th>Concentration </th></tr><tr><td>S04617 + EFE ligation 1:2 sample 1</td><td>459 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 2</td><td>603.2 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 3</td><td>294.2 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 1</td><td>434.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 2</td><td>346.3 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 1</td><td> 351.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 2</td><td>293.7 ng/&micro;l</td></tr></table></center></html>}}<html> We proceeded then performing a screening </del>digestion <del class="diffchange diffchange-inline">followed by an electrophoresis analysis. Kapa universal ladder was adopted.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/02/Tn2013_GEL_aracpbad-EFE_S04617%2BEFE.jpg\"></center></html>}}<html> As you can see from the image, AraCpBAD </del>+ <del class="diffchange diffchange-inline">EFE (BBa_K1065002) part is confirmed for all the 3 samples (lane 1 to 3</del>, <del class="diffchange diffchange-inline">2426 bp). Differently, BBa_S04617 + EFE (BBa_K1265002) is not confirmed (lane 5 </del>to <del class="diffchange diffchange-inline">7, 2460 bp expected) since </del>the <del class="diffchange diffchange-inline">band is at an heigh of about 1400 bp</del>. <del class="diffchange diffchange-inline">Probably </del>the <del class="diffchange diffchange-inline">destination vector BBa_S04617 was closed itself without incorporating EFE</del>.</html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "content" : "<html><ins class="diffchange diffchange-inline">Today </ins>we performed a digestion <ins class="diffchange diffchange-inline">of J23100</ins>+<ins class="diffchange diffchange-inline">K592016 with EcoR I and Spe I</ins>, <ins class="diffchange diffchange-inline">and K592020 in pSB1C3 with EcoR I and XbaI </ins>to <ins class="diffchange diffchange-inline">obtain </ins>the <ins class="diffchange diffchange-inline">complete biobrick</ins>.<ins class="diffchange diffchange-inline">..waiting tomorrow for </ins>the <ins class="diffchange diffchange-inline">results</ins>.</html>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "tags" : "<del class="diffchange diffchange-inline">pFixK-cI-pLambda-EFE</del>-<del class="diffchange diffchange-inline">AraCpBAD</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "tags" : "<ins class="diffchange diffchange-inline">K592016</ins>-<ins class="diffchange diffchange-inline">K592020</ins>"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
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Ggirelli
http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/08/21&diff=110261&oldid=prev
Ggirelli: moved Team:UNITN-Trento/Notebook/Labposts/157 to Team:UNITN-Trento/Notebook/Labposts/08/21
2013-09-09T07:34:43Z
<p>moved <a href="/Team:UNITN-Trento/Notebook/Labposts/157" class="mw-redirect" title="Team:UNITN-Trento/Notebook/Labposts/157">Team:UNITN-Trento/Notebook/Labposts/157</a> to <a href="/Team:UNITN-Trento/Notebook/Labposts/08/21" title="Team:UNITN-Trento/Notebook/Labposts/08/21">Team:UNITN-Trento/Notebook/Labposts/08/21</a></p>
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<td colspan='1' style="background-color: white; color:black;">← Older revision</td>
<td colspan='1' style="background-color: white; color:black;">Revision as of 07:34, 9 September 2013</td>
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Ggirelli
http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/08/21&diff=78673&oldid=prev
Ggirelli: Created page with "{ "date" : "2013-08-12", "author" : "thomas-viola-michele", "title" : "Minipreps and screening", "content" : "<html>Starting from the o/n cultures prepared <a href=\"http://2..."
2013-08-19T13:54:03Z
<p>Created page with "{ "date" : "2013-08-12", "author" : "thomas-viola-michele", "title" : "Minipreps and screening", "content" : "<html>Starting from the o/n cultures prepared <a href=\"http://2..."</p>
<p><b>New page</b></p><div>{<br />
"date" : "2013-08-12",<br />
"author" : "thomas-viola-michele",<br />
"title" : "Minipreps and screening",<br />
"content" : "<html>Starting from the o/n cultures prepared <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-11-thomas-viola\">yesterday</a>, we performed a miniprep purification, obtaining these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><table><tr><th>Sample</th><th>Concentration </th></tr><tr><td>S04617 + EFE ligation 1:2 sample 1</td><td>459 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 2</td><td>603.2 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 3</td><td>294.2 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 1</td><td>434.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 2</td><td>346.3 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 1</td><td> 351.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 2</td><td>293.7 ng/&micro;l</td></tr></table></center></html>}}<html> We proceeded then performing a screening digestion followed by an electrophoresis analysis. Kapa universal ladder was adopted.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/02/Tn2013_GEL_aracpbad-EFE_S04617%2BEFE.jpg\"></center></html>}}<html> As you can see from the image, AraCpBAD + EFE (BBa_K1065002) part is confirmed for all the 3 samples (lane 1 to 3, 2426 bp). Differently, BBa_S04617 + EFE (BBa_K1265002) is not confirmed (lane 5 to 7, 2460 bp expected) since the band is at an heigh of about 1400 bp. Probably the destination vector BBa_S04617 was closed itself without incorporating EFE.</html>",<br />
"tags" : "pFixK-cI-pLambda-EFE-AraCpBAD"<br />
}</div>
Ggirelli