Team:UNITN-Trento/Notebook/Labposts/08/40

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Latest revision as of 09:13, 3 October 2013

{ "date" : "2013-08-15", "author" : "viola", "title" : "Kg of DNA!!!", "content" : "Yesterday i made some inocula from the plates of the sequenced clones transformation (pXyl+EFE+B0015 and pSpac+EFE+B0015). Today i made some minipreps and i made also a screening cutting with SpeI (i made a little mess with the minicolumns...ehm...). This is the gel:

Gel Image

", "tags" : "pSpac-pXyl-EFE-B0015" }

@@ Line 1: / Line 1: @@
-{"date" : "2013-08-31","author" : "thomas","title" : "<html>Sequencing and reamplification of AraC-pBAD EFE</html>","content" : "<html>This morning I had to reamplify the construnct AraC-pBAD EFE in order to have a stock of it. So I starting from the inocula previously prepared, I did miniprep purification and quantification of six samples obtaining the following concentrations.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th>Sample</th><th>Concentration (ng/&micro;l)</th></tr><tr><td>1:1 sample 1</td><td>383</td></tr><tr><td>1:1 sample 2</td><td>174.8</td></tr><tr><td>1:1 sample 3</td><td>182.4</td></tr><tr><td>1:2 sample 1</td><td>173.9</td></tr><tr><td>1:2 sample 2</td><td>154.3</td></tr><tr><td>1:2 sample 3</td><td>188</td></tr></table></html>}}<html> The construct was successfully reamplified!</html>","tags" : "aracpbad-efe"}
+{
+	"date" : "2013-08-15",
+	"author" : "viola",
+	"title" : "Kg of DNA!!!",
+	"content" : "<html>Yesterday i made some inocula from the plates of the sequenced clones transformation <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-13-viola\">(pXyl+EFE+B0015 and pSpac+EFE+B0015)</a>. Today i made some minipreps and i made also a screening cutting with SpeI (i made a little mess with the minicolumns...ehm...). This is the gel:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://2013.igem.org/File:Tn-2013-Xylespacgiusti.JPG\"/></center></html>}}<html> </html>",
+	"tags" : "pSpac-pXyl-EFE-B0015"
+}