Team:UNITN-Trento/Notebook/Labposts/08/45

From 2013.igem.org

(Difference between revisions)
 
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{
{
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"date" : "2013-08-17",
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"date":"2013-08-16",
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"author" : "emil",
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"author":"viola-caterina",
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"title" : "To be continued 2 ",
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"title":"<html>A night in da (C)LAB</html>",
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"content" : "<html> I verified that the transformation has succeded only in one case (1:1) with 4 colonies so I performed the miniprep following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep'> protocol </a>, after that I tested the construct by digestion with Sca1 following the Thermo scientific  protocol for Sca1 digestion  with the following result:</html> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src='https://static.igem.org/mediawiki/2013/b/b8/Tn-2013_Foto_%288%29.JPG' width='400px;'></html>}}<html> as you can see in the second lane ther is a single  bright band at 6000 bp which correspond to the final construct, this construct were transformed in 400 &micro;l <i>Bacillus</i> cells growth at O.D. 1.1 in Minimal medium following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'> transformation protocol </a>. These cells were plated on Spectinomicin added Agar.</html>",
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"content":"<html>We passed a really beautiful night in the lab starting a cloning short protocol at 19:30 and plating the cells at 5:00 am.... we are really mad!!!!! </html>",
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"tags" : "pXyl-GFP"
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"tags":"EFE"
}
}

Latest revision as of 09:15, 3 October 2013

{ "date":"2013-08-16", "author":"viola-caterina", "title":"A night in da (C)LAB", "content":"We passed a really beautiful night in the lab starting a cloning short protocol at 19:30 and plating the cells at 5:00 am.... we are really mad!!!!! ", "tags":"EFE" }