Team:UNITN-Trento/Notebook/Labposts/08/47

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(Difference between revisions)

Latest revision as of 09:16, 3 October 2013

{ "date" : "2013-08-18", "author" : "thomas", "title" : "Preparing sequencing samples of AraC-pBAD EFE", "content" : "This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:

Quantification results

	Volume added to sample 1:1 (µl)	Volume added to sample 1:2 (µl)
Template DNA	5.5	6.5
Primer	2	2
Water	up to 15	up to 15

The samples were then putted in a falcon and send with a courier. ", "tags" : "aracpbad-efe" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-20",
+	"date" : "2013-08-18",
-	"author" : "bruno",
+	"author" : "thomas",
-	"title" : "<html>Mutagenesis, the revenge</html>",
+	"title" : "<html>Preparing sequencing samples of AraC-pBAD EFE</html>",
-	"content" : "<html>After the creation of K1065300 (without RBS) I doing tha mutagenesis of this part with 67 degree and 1.5 ul of DMSO.</html>",
+	"content" : "<html>This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers.  So knowing the preps concentrations, I prepared the mix as follow:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th></th><th>Volume added to sample 1:1 (&micro;l)</th><th>Volume added to sample 1:2 (&micro;l)</th></tr><tr><th>Template DNA</th><td>5.5</td><td>6.5</td></tr><tr><th>Primer</th><td>2</td><td>2</td></tr><tr><th>Water</th><td>up to 15</td><td>up to 15</td></tr></table></html>}}<html> The samples were then putted in a falcon and send with a courier. </html>",
-	"tags" : "Blue light, mutagenesis"
+	"tags" : "aracpbad-efe"
 }