http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/08/56&feed=atom&action=historyTeam:UNITN-Trento/Notebook/Labposts/08/56 - Revision history2024-03-28T12:41:50ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/08/56&diff=273616&oldid=prevGgirelli at 09:18, 3 October 20132013-10-03T09:18:11Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:18, 3 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "date" : "2013-08-<del class="diffchange diffchange-inline">03</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "date":"2013-08-<ins class="diffchange diffchange-inline">31</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "author" : "<del class="diffchange diffchange-inline">gabriele</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "author":"<ins class="diffchange diffchange-inline">fabio</ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "title" : "<del class="diffchange diffchange-inline">Wintergreen with pLac-SAMsynthetase: Ep. II</del>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "title":" <ins class="diffchange diffchange-inline"><html> the blue circuit doesn’t always work!!</html> </ins>",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "content" : "<html>I <del class="diffchange diffchange-inline">miniprepped </del>the <del class="diffchange diffchange-inline">six inocula from yesterday (great yields, minimum is 249.9 ng/&micro;l) and screened them </del>with <del class="diffchange diffchange-inline">EcoRI-HF </del>and <del class="diffchange diffchange-inline">PstI-HF. One sample was positive! YEAH!!! </html>{{</del>:<del class="diffchange diffchange-inline">Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>13A</td></tr><tr><td>13B</td></tr><tr><td>13C</td></tr><tr><td><i>Empty</i></td></tr><tr><td>14A</td></tr><tr><td>14B</td></tr><tr><td>14C</td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static</del>.<del class="diffchange diffchange-inline">igem</del>.<del class="diffchange diffchange-inline">org/mediawiki/2013/f/f7/Tn-20130803_SAMinWGREEN_check_2</del>.<del class="diffchange diffchange-inline">jpg\" /></html>}}<html>Being completely honest I admit that from </del>the <del class="diffchange diffchange-inline">first gel the result was </del>not <del class="diffchange diffchange-inline">clear, so </del>I <del class="diffchange diffchange-inline">performed </del>a <del class="diffchange diffchange-inline">second electroforesis run </del>with a <del class="diffchange diffchange-inline">ctrl (wintergreen alone, digested </del>with <del class="diffchange diffchange-inline">EcoRI-HF </del>and <del class="diffchange diffchange-inline">PstI-HF). In </del>this <del class="diffchange diffchange-inline">last screen it is clear how 13A is positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>1kb ladder</td></tr><tr><td>13A</td></tr><tr><td>Ctrl</td></tr><tr><td><i>14B</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static</del>.<del class="diffchange diffchange-inline">igem</del>.<del class="diffchange diffchange-inline">org/mediawiki/2013/7/76/Tn-20130803_SAMinWGREEN_check_3.jpg\" /></html>}}<html></del></html>",</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "content":" <html> I <ins class="diffchange diffchange-inline">repeated </ins>the <ins class="diffchange diffchange-inline">experiment </ins>with <ins class="diffchange diffchange-inline">light induction lots </ins>and <ins class="diffchange diffchange-inline">lots of times, but unfortunately a few time cells behaved strangely</ins>: <ins class="diffchange diffchange-inline">even the dark control produced amilCP</ins>... <ins class="diffchange diffchange-inline">probably </ins>the <ins class="diffchange diffchange-inline">circuit is </ins>not <ins class="diffchange diffchange-inline">perfectly regulated!! Now </ins>I <ins class="diffchange diffchange-inline">have to think about </ins>a <ins class="diffchange diffchange-inline">new cloning: pedro will try out a ligation of the circuit </ins>with <ins class="diffchange diffchange-inline">EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do </ins>a <ins class="diffchange diffchange-inline">PCR of the improved part </ins>with <ins class="diffchange diffchange-inline">Kapa polymesase </ins>and <ins class="diffchange diffchange-inline">then inserting </ins>this <ins class="diffchange diffchange-inline">sequence inside the plasmid containing EFE +terminators</ins>. <ins class="diffchange diffchange-inline">Then we will have ethylene production upon illumination</ins>.</html> ",</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> "tags" : "<del class="diffchange diffchange-inline">Wintergreen-SAMsynthetase</del>-<del class="diffchange diffchange-inline">pLac</del>"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> "tags":"<ins class="diffchange diffchange-inline">blue_light</ins>-<ins class="diffchange diffchange-inline">EFE</ins>"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
</table>Ggirellihttp://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook/Labposts/08/56&diff=266978&oldid=prevGgirelli: Created page with "{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "<html>I miniprepped the six inocula from yesterday (grea..."2013-10-02T13:07:31Z<p>Created page with "{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "<html>I miniprepped the six inocula from yesterday (grea..."</p>
<p><b>New page</b></p><div>{<br />
"date" : "2013-08-03",<br />
"author" : "gabriele",<br />
"title" : "Wintergreen with pLac-SAMsynthetase: Ep. II",<br />
"content" : "<html>I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/&micro;l) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!! </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>13A</td></tr><tr><td>13B</td></tr><tr><td>13C</td></tr><tr><td><i>Empty</i></td></tr><tr><td>14A</td></tr><tr><td>14B</td></tr><tr><td>14C</td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/f/f7/Tn-20130803_SAMinWGREEN_check_2.jpg\" /></html>}}<html>Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>1kb ladder</td></tr><tr><td>13A</td></tr><tr><td>Ctrl</td></tr><tr><td><i>14B</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/7/76/Tn-20130803_SAMinWGREEN_check_3.jpg\" /></html>}}<html></html>",<br />
"tags" : "Wintergreen-SAMsynthetase-pLac"<br />
}</div>Ggirelli