Team:UNITN-Trento/Notebook/Labposts/08/57

From 2013.igem.org

(Difference between revisions)
(Created page with "{ "date" : "2013-08-07", "author" : "gabriele-emil", "title" : "M9 Minimal Medium", "content" : "<html>Simply (it is not very easy but we succesed) prepared the M9 minimal me...")
 
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{
{
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"date" : "2013-08-07",
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"date" : "2013-08-31",
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"author" : "gabriele-emil",
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"author" : "thomas",
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"title" : "M9 Minimal Medium",
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"title" : "<html>Sequencing and reamplification of AraC-pBAD EFE</html>",
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"content" : "<html>Simply (it is not very easy but we succesed) prepared the M9 minimal medium!</html>",
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"content" : "<html>This morning I had to reamplify the construnct AraC-pBAD EFE in order to have a stock of it. So I starting from the inocula previously prepared, I did miniprep purification and quantification of six samples obtaining the following concentrations.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th>Sample</th><th>Concentration (ng/&micro;l)</th></tr><tr><td>1:1 sample 1</td><td>383</td></tr><tr><td>1:1 sample 2</td><td>174.8</td></tr><tr><td>1:1 sample 3</td><td>182.4</td></tr><tr><td>1:2 sample 1</td><td>173.9</td></tr><tr><td>1:2 sample 2</td><td>154.3</td></tr><tr><td>1:2 sample 3</td><td>188</td></tr></table></html>}}<html> The construct was successfully reamplified!</html>",
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"tags" : "M9"
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"tags" : "aracpbad-efe"
}
}

Latest revision as of 09:18, 3 October 2013

{ "date" : "2013-08-31", "author" : "thomas", "title" : "Sequencing and reamplification of AraC-pBAD EFE", "content" : "This morning I had to reamplify the construnct AraC-pBAD EFE in order to have a stock of it. So I starting from the inocula previously prepared, I did miniprep purification and quantification of six samples obtaining the following concentrations.

Quantification results
SampleConcentration (ng/µl)
1:1 sample 1383
1:1 sample 2174.8
1:1 sample 3182.4
1:2 sample 1173.9
1:2 sample 2154.3
1:2 sample 3188
The construct was successfully reamplified!", "tags" : "aracpbad-efe" }