Team:USTC CHINA

From 2013.igem.org

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<h1>Design of Project</h1>
<h1>Design of Project</h1>
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   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.</div>
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   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.</div>
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<div id="ls" class="pro-col">
   <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
   <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
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   <span>ANTIGEN</span><br />
   <span>ANTIGEN</span><br />
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid".
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <span>LTB</span><br />
   <span>LTB</span><br />
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
<br />
<br />
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <span>TNFα</span><br />
   <span>TNFα</span><br />
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TNFαis a chemotactic factor that recruits Langerhans Cells, antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for immigrating LC and triggering more intense immune responses.
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TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
<br />
<br />
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <div id="pro-de-text">
   <span>REPORTER</span><br />
   <span>REPORTER</span><br />
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Reporter notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is conveyed in them as a biosafety guard.
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Reporter notifies users the status of vaccine patch and when to stick.  Furthermore, it contains killing switch as a biosafety guard.
<br />
<br />
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <div id="exp-text">
   <div id="exp-text">
   <span>Tests on fusion protein</span><br />
   <span>Tests on fusion protein</span><br />
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   To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verified design.<br />
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   To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br />
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
   </div>
   </div>

Revision as of 04:19, 19 October 2013

USTC_China

In Situ Transdermal Vaccine

A revolutionary transdermal vaccine delivery
Standardized in situ expression system
Safe, Reliable, Needle-free and Available for everyone
2013, we redefined vaccine

Project

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
more

Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will make up great percentage of
in the total bacteria in our "band-aid".
more
LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
more
TNFα
TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
more
REPORTER
Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard.
more

Experimental Measurement

Construction of Vectors
We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.
more
Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.
more
Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more

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