Team:USTC CHINA/Modeling

From 2013.igem.org

(Difference between revisions)
(Prototype team page)
 
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{USTC-China/hidden}}
-
 
+
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<head>
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/main.css?action=raw&ctype=text/css" />
-
This is a template page. READ THESE INSTRUCTIONS.
+
</head>
 +
<body background="https://static.igem.org/mediawiki/2013/6/62/2013ustc-china_Light_grey_bg.png">
 +
<div class="bar" align="center">
 +
    <div class="container" align="left">
 +
<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div>
 +
 
 +
<ul id="nav">
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
 +
            <ul class="subs">
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
 +
              <ul class="subs">
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
 +
              </ul>
 +
        </li>
 +
        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
 +
                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
 +
                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
 +
    </ul>
 +
        <div id="tlogo"><img src="https://static.igem.org/mediawiki/2013/f/f8/2013ustc-china_T-VACCINE.png" width="100%" height="123" />
 +
        </div>
 +
        </div>
 +
        </div>
 +
<div class="content" align="center">
 +
<div class="conbar1">
 +
<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a></div></div>
 +
<div class="conbar2">
 +
<div class="leftbar" align="left" style="margin-bottom:30px;">
 +
<div style="margin-top:20px;">
 +
<h1>Modeling on the suicide mechanism of the reporter system</h1>
 +
<p>To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine.
 +
Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.</p>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">read more about Kill Switch</a>
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
 
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
<br><br>
 +
<div>
 +
<h1>The best growing condition for Bacillus subtilis WB800N</h1>
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
<div>
-
You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.   
+
To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for Bacillus subtilis WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:
 +
<table width="400" border="1px"
 +
<tr>
 +
  <th align="left">Factor</th>
 +
  <th align="right">Low</th>
 +
  <th align="right">High</th>
 +
  </tr>
 +
  <tr>
 +
  <td align="left">Temperature</td>
 +
  <td align="right">25℃</td>
 +
  <td align="right">35℃</td>
 +
</tr>
 +
<tr>
 +
  <td align="left">Time</td>
 +
  <td align="right">12h</td>
 +
  <td align="right">24h</td>
 +
</tr>
 +
<tr>
 +
  <td align="left">Peptone</td>
 +
  <td align="right">5</td>
 +
  <td align="right">15</td>
 +
</tr>
 +
<tr>
 +
  <th align="left">Yeast Extract</th>
 +
  <th align="right">2.5</th>
 +
  <th align="right">7.5</th>
 +
  </tr>
 +
<tr>
 +
  <th align="left">NaCl</th>
 +
  <th align="right">5</th>
 +
  <th align="right">15</th>
 +
</tr>
 +
</table>
 +
 
</div>
</div>
 +
<br><br>
 +
<div>
 +
 +
Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis.
 +
<br>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">read more about B.Subtilis Culture</a>
</div>
</div>
-
</html>
 
-
<!-- *** End of the alert box *** -->
+
<br><br>
 +
<div>
 +
<h1>Designs of Immune Experiments</h1>
 +
<p>Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineering competition than a science competition sometimes. </p>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">read more about Designs of Immune Experiments</a>
 +
</div>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
-
!align="center"|[[Team:USTC_CHINA|Home]]
 
-
!align="center"|[[Team:USTC_CHINA/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA Official Team Profile]
 
-
!align="center"|[[Team:USTC_CHINA/Project|Project]]
 
-
!align="center"|[[Team:USTC_CHINA/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:USTC_CHINA/Modeling|Modeling]]
 
-
!align="center"|[[Team:USTC_CHINA/Notebook|Notebook]]
 
-
!align="center"|[[Team:USTC_CHINA/Safety|Safety]]
 
-
!align="center"|[[Team:USTC_CHINA/Attributions|Attributions]]
 
-
|}
 
 +
</div>
-
If you choose to include a '''Modeling''' page, please write about your modeling adventures here. This is not necessary but it may be a nice list to include.
+
<div class="rightbar">
 +
<div class="port-sidebar-border"><h>Modeling</h></div>
 +
<div class="clear"></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs Of Immune Experiments</a></div>
 +
</div></div></div>
 +
   
 +
</body>
 +
</html>

Latest revision as of 22:37, 27 September 2013

Modeling on the suicide mechanism of the reporter system

To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine. Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.

read more about Kill Switch


The best growing condition for Bacillus subtilis WB800N

To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for Bacillus subtilis WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:
Factor Low High
Temperature 25℃ 35℃
Time 12h 24h
Peptone 5 15
Yeast Extract 2.5 7.5
NaCl 5 15


Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis.
read more about B.Subtilis Culture


Designs of Immune Experiments

Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineering competition than a science competition sometimes.

read more about Designs of Immune Experiments
Modeling

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Modeling"