Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis


Expression of proteins in Bacillus subtilis

Induction with IPTG
- grow appropriate B. subtilis strain overnight in fresh 2xYT medium
- inoculate into fresh 2xYT medium to an OD600 of 0.15
- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM
IPTG(CB-0487-1GAM) to one portion (t = 0)
- collect samples at different time points for analysis (t = 1,…)