Team:USTC CHINA/Parts

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TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis...