Team:UTK-Knoxville

From 2013.igem.org

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div.MenuBar ul li a:hover ul.DropDownMenu li a {
-
  height: 656px;
+
   background-color: Menu; Drop-down menu unselected items.
-
   top: 303px;
+
                            Sets the drop-down menu "effective background" color. */
-
   left: 72px;
+
   color: MenuText;
}
}
-
#absolute-wrapper .div-2.div-4 .dom-heading {
+
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
-
   font-family:"Arial";
+
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
-
   text-align: center;
+
   background-color: Highlight; /* Drop-down menu selected item. */
 +
   color: HighlightText;
}
}
-
#absolute-wrapper .dom-link {
+
/*...............................................................................................*/
-
  background: #ffffff;
+
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
-
  color: #f77f00;
+
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
-
  border-radius: 10px;
+
   background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
-
  border: 1px solid #4c4d4f;
+
-
   font-size: 18px;
+
-
  text-align: center;
+
-
  padding: 5px;
+
-
  width: 213px;
+
-
  height: 43px;
+
-
  position: absolute;
+
-
  top: 341px;
+
-
  left: 642px;
+
}
}
-
#absolute-wrapper .image.image-1.image-3 {
+
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
-
  width: 150px;
+
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
-
  height: 100px;
+
   background-color: Menu; /* Side menu unselected items.
-
   top: 418px;
+
                            Sets the side menu "effective background" color. */
-
   left: 686px;
+
   color: MenuText;
}
}
-
#absolute-wrapper .div-2.div-3.div-5 {
+
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
-
   top: 568px;
+
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
-
   left: 591px;
+
   background-color: Highlight; /* Side menu selected item. */
 +
   color: HighlightText;  
}
}
-
#absolute-wrapper .div-2.div-3.div-5 p {
+
/*-----------------------------------------------------------------------------------------------*/
-
   font-family:"helvetica";
+
/*Script-Free 3-Level Menu 1.2 Tailor
-
   font-size: 40px;
+
  www.CesarDaniel.info
-
   text-align: center;
+
/*-------------------------------------------------------------------------------------- General */
 +
body {
 +
   background: white;
 +
   color: black;
 +
   margin: 0;
 +
  border: 0;
 +
  padding: 0;
}
}
-
#absolute-wrapper .div-2.div-3.div-5 .dom-heading {
+
 
-
   font-family:"Arial";
+
 
-
  text-align: center;
+
div.MenuBar {
 +
   font: 13px arial, helvetica, sans-serif;
}
}
-
#absolute-wrapper .div-2.div-3.div-6 {
+
div.MenuBar ul {
-
   top: 806px;
+
   font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
-
  left: 600px;
+
}
}
-
#absolute-wrapper .div-2.div-3.div-6 .image {
+
/*--------------------------------------------------------------------------------------- Colors */
-
   border-radius: 10px;
+
div.MenuBar {
 +
  background-color: black; /* Selected item. */
 +
  color: #FFFFFF;
 +
   border-bottom: 1px solid ButtonShadow;
}
}
-
.container {
+
div.MenuBar a,
-
   height: 258px;
+
div.MenuBar ul li:hover ul.DropDownMenu li a,
-
   width: 940px;
+
div.MenuBar ul li a:hover ul.DropDownMenu li a,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
 +
   background-color: black; /* Selected item. */
 +
   color: #FFFFFF;
}
}
-
.container .easel-inserted-link .image {
+
div.MenuBar ul li:hover a,
-
   border-radius: 10px;
+
div.MenuBar ul li a:hover,
-
   margin-top: 10px;
+
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
 +
   background-color: #00b0e6; /* Selected item. */
 +
   color: #FFFFFF;
}
}
 +
div.MenuBar ul li:hover ul.DropDownMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
 +
  background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
 +
}
 +
/*--------------------------------------------------------------------------------------- Widths */
 +
/*
 +
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
 +
  width: 11em; /* Drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
 +
  width: 12em; /* Drop-down menu width. */
 +
}
 +
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #1
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
 +
  left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #2
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
 +
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #3
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
 +
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
 +
</style>
 +
 +
 +
 +
</p><div id="header"><a href="https://2013.igem.org"><img src="https://lh5.googleusercontent.com/-HN_AgwewsJM/UZW60twqf3I/AAAAAAAAAA8/R7NTt_xL13c/w910-h253-no/header.jpg" alt="Team UTK-Knoxville"></a></div>
 +
<div class="MenuBar" id="navi">
 +
        <ul>
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Home
 +
                        <!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                        <ul class="DropDownMenu" id="MB1-DDM6">
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Advisors</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Undergraduates</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>University</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION5</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION6</span></span></a></li>
 +
                        </ul>
 +
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                        <ul class="DropDownMenu" id="MB1-DDM1">
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Material &amp; Methods</span></span></a>
 +
                        </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
 +
                        </ul>
 +
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                <ul class="DropDownMenu" id="MB1-DDM2">
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Submitted Parts</span></span></a></li>
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li>
 +
                                </ul>
 +
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Background<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                <ul class="DropDownMenu" id="MB1-DDM3">
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li>
 +
                                </ul>
 +
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
                <li>
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                <ul class="DropDownMenu" id="MB1-DDM5">
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                        <ul class="SideMenu" id="MB1-DDM2-SM1">
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION2</span></a></li>
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION3</span></a></li>
 +
                                        </ul>
 +
                                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                                </li>
 +
                               
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxvillecomingsoon"><span><span>SECTION4 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                        <ul class="SideMenu" id="MB1-DDM2-SM2">
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION8</span></a></li>
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION9</span></a></li>
 +
                                        </ul>
 +
                                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                                </li>
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION10 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                        <ul class="SideMenu" id="MB1-DDM2-SM3">
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION11</span></a></li>
 +
                                        <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION12</span></a></li>
 +
                                        </ul>
 +
                                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                                </li>
 +
                                <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Visualization</span></span></a>
 +
                                </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Material &amp; Methods</span></span></a>
 +
                                </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a>
 +
                                </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a>
 +
                                </li></ul>
 +
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
                </li>
 +
                <li style="width: 160px">
 +
                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Safety<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
                                <ul class="DropDownMenu" id="MB1-DDM4">
 +
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li>
 +
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section1</span></span></a></li>
 +
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
 +
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li>
 +
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section4</span></span></a></li>
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                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section5</span></span></a></li>                           
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                </li>         
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                <li>
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                        <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Sponsors</a>
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                </li>
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        </ul>
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<p></p><p><br>
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</p><p><br>
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</p>
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<h2> <span class="mw-headline" id="Background">Abstract</span></h2>
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<p>The major limitation in synthetic biology today is the lack of numerous, well characterized sensors.  Our project aims to provide a reliable scaffold to test potential sensing domains with unknown substrates.  We have created a standard platform to test a range of intracellular and transmembrane domains.  Positive results are reported with green fluorescent protein for easy identification which can be done with high throughput methods like 96 well plates.  We test our platform on sensors with interesting known responses.  The chimera proteins are also useful in creating signals orthogonal to the cell. <br>
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[<a href="https://2013.igem.org/Team:UTK-Knoxville/home" class="external text" rel="nofollow">back</a>]
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<h2>&nbsp;</h2><p>&nbsp;</p>
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Revision as of 20:34, 18 June 2013

Team:UTK-Knoxville - 2013.igem.org

Team:UTK-Knoxville

2013.igem.org



Abstract

The major limitation in synthetic biology today is the lack of numerous, well characterized sensors. Our project aims to provide a reliable scaffold to test potential sensing domains with unknown substrates. We have created a standard platform to test a range of intracellular and transmembrane domains. Positive results are reported with green fluorescent protein for easy identification which can be done with high throughput methods like 96 well plates. We test our platform on sensors with interesting known responses. The chimera proteins are also useful in creating signals orthogonal to the cell.
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