Team:UTK-Knoxville/11 June 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"><head> <title>Team:UK-Knoxville - 2013.igem.org</title> <link rel="stylesheet" type="text/css" me...")
Line 551: Line 551:
</style>
</style>
<h3> <span class="mw-headline" id="Tuessday_11_June_13"> Tuesday 11 June 13 </span>      </h3>
<h3> <span class="mw-headline" id="Tuessday_11_June_13"> Tuesday 11 June 13 </span>      </h3>
 +
<p>Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any.
 +
 +
Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before today’s 5:00 meeting.
 +
 +
Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent ΔOmpR, ΔEnvZ, and ΔOmpR+ΔEnvZ transform our plasmid into.</p>
<li> Whole group meeting </li>
<li> Whole group meeting </li>
<li> Aer/EnvZ is in the wild type.</li>
<li> Aer/EnvZ is in the wild type.</li>

Revision as of 10:39, 6 July 2013

Team:UK-Knoxville - 2013.igem.org

2013.igem.org

Tuesday 11 June 13

Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any. Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before today’s 5:00 meeting. Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent ΔOmpR, ΔEnvZ, and ΔOmpR+ΔEnvZ transform our plasmid into.

  • Whole group meeting
  • Aer/EnvZ is in the wild type.
  • Keep the website broad for now. But keep the offline files detailed.
  • Need to get the mutants for the cytoplasmic platform.
  • Can grow E coli on nitrates under anaerobic conditions as a negative control.
  • Design characterization experiments and controls for each construct.
  • For outreach on June 14th - videos, photos, short write up, demonstrations / activities done...
    • [Back to Notebook]

    Retrieved from "http://2013.igem.org/Team:UTK-Knoxville/11_June_2013"