http://2013.igem.org/wiki/index.php?title=Team:UTK-Knoxville/28_June_2013&feed=atom&action=history
Team:UTK-Knoxville/28 June 2013 - Revision history
2024-03-29T05:49:17Z
Revision history for this page on the wiki
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http://2013.igem.org/wiki/index.php?title=Team:UTK-Knoxville/28_June_2013&diff=25694&oldid=prev
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2013-07-06T10:13:47Z
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<div id="header"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/thumb/2/20/Header2.jpg/800px-Header2.jpg" alt="Team UTK-Knoxville" width="910" height="234"></a></div><br />
<div class="MenuBar" id="navi"><br />
<ul><br />
<li><br />
<a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Home<br />
<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--><br />
<!--[if lte IE 6]></td></tr></table></a><![endif]--><br />
</li><br />
<li><br />
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Project<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--><br />
<ul class="DropDownMenu" id="MB1-DDM1"><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a><br />
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li><br />
</ul><br />
<!--[if lte IE 6]></td></tr></table></a><![endif]--><br />
</li><br />
<li><br />
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--><br />
<ul class="DropDownMenu" id="MB1-DDM2"><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/Aer"><span>Aer</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>HemAT</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>RcoM</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>NarX</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>TodS</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>LasR</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(EnvZ)</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(NtrC)</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/primers"><span>Primers</span></a></li><br />
</ul><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li><br />
</ul><br />
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<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Discussion<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<ul class="DropDownMenu" id="MB1-DDM3"><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li><br />
</ul><br />
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<a href="https://2013.igem.org/Team:UTK-Knoxville/notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span>SECTION2</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION3</span></a></li><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxvillecomingsoon"><span><span>SECTION4 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION8</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION9</span></a></li><br />
</ul><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION10 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION11</span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION12</span></a></li><br />
</ul><br />
<!--[if lte IE 6]></td></tr></table></a><![endif]--><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Visualization</span></span></a><br />
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a><br />
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a><br />
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a><br />
</li></ul><br />
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<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">oUTreach<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<ul class="DropDownMenu" id="MB1-DDM4"><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section1</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section4</span></span></a></li><br />
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section5</span></span></a></li> <br />
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<a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]--><br />
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/undergraduates"><span><span>Undergraduates</span></span></a></li><br />
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<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a><br />
</li><br />
</ul><br />
<br />
</div><br />
<h1><span class="mw-headline">June 28th, 2013</span></h1><br />
<p>We now have stock of Azo-Tray 14, Box 1, Well 53.<br />
<br />
Patrick - Set up a 25 uL Q5 master mix PCR reaction to extract HemAT from the R. Leg gDNA. Used HemAT_F and HemAT_R with a Tm of 52 and 56, an elongation time of 1:15 minutes, and 32 cycles. This is the Tm established by our analysis with APE when creating the primers. The Tm from the sides of the primer tubes would suggest a Tm of 60 C but this is taking into account the full length of the primer (including the overhang). HemAT is 558 bp and the master mix has an extension of 30 seconds / kb. 30-35 cycles is recommended for gDNA amplification. I used 2 uL of template DNA (~50 ng). Tomorrow Eric will run a gel and isolate HemAT.<br />
<br />
I planned to make stock of R. Leg today, but it did not grow while I was gone, so I am growing more in the 37C shaker and will make stock Monday.<br />
<br />
Brandon ran a gel on Patrick’s PCR of HemAT and RcoM. The PCR did not work, so he has set up a PCR at a range of temps(56, 60, 64) see if the low temperature is causing the problem.</p><br />
<p><br><br />
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