Team:UTK-Knoxville/30 May 2013

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Thursday 30 May 13

  • Amplification of Aer, EnvZ, and OmpR from MG1655 gDNA:
    • Aer:
    • primers: Aer_f and Aer-EnvZ_1r
    • Tm: 52 and 55 (both worked the same, so lets use 55) Expected length: about 860bp
    • EnvZ
    • primers: Aer_f and Aer-EnvZ_1r
    • Tm: 57 and 60 (both worked the same, so lets use 60)
    • Expected length: about 650bp
    • OmpR
    • primers: OmpR_r and OmpR_f
    • Tm: 60
    • Expected length: 720bp
    • Julie ran the gel and they all looked good.
    • Overlapping PCR of Aer/EnvZ
    • primers: Aer_f and EnvZ_r
    • expected length: 1490bp
    • The gel was kind of streaky because Julie changed something on the machine Julie shouldn’t have, but the band we wanted was still there. The Team had a fiasco with the gel kit though. Julie ran it back through a gel to see if it was still there and it wasn’t, so we need to redo that.
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