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Team:UT Dallas/week4
From 2013.igem.org
(Created page with "<p class="c2"><span>7/22/2013</span></p><p class="c2"><span>[GFP] – I3-5-8 Carb Resistance</span></p><p class="c2"><span>[pLac]- I5-6-3 Chlora</span></p><p class="c2"><span>I4-...") |
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<p class="c2"><span>7/22/2013</span></p><p class="c2"><span>[GFP] – I3-5-8 Carb Resistance</span></p><p class="c2"><span>[pLac]- I5-6-3 Chlora</span></p><p class="c2"><span>I4-45-2 Chlora</span></p><p class="c1"><span></span></p><p class="c2"><span>7/23/2013</span></p><p class="c2"><span>Transformation Results:</span></p><a href="#" name="561d1c16c2e9d617f036d365af80fddf4eda0c9f"></a><a href="#" name="5"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c0"><p class="c1 c4"><span></span></p></td><td class="c0"><p class="c4 c2"><span># of colonies</span></p></td><td class="c0"><p class="c1 c4"><span></span></p></td></tr><tr class="c8"><td class="c0"><p class="c1 c4"><span></span></p></td><td class="c0"><p class="c4 c2"><span>NEB</span></p></td><td class="c0"><p class="c4 c2"><span>Commercial </span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>I4-45-2</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>0</span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>I5-6-3 [pLac]</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>90+</span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>GFP-I3-5-8</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>Tmtc</span></p></td></tr></tbody></table><p class="c1"><span></span></p><p class="c2"><span>[pLac]-I5-6-3 → [NEB] 100+ colonies → picked two colonies → i5-10-3 </span></p><p class="c2"><span> → i5-10-4</span></p><p class="c2"><span>[GFP]-I3-5-8 → [NEB] 100+ colonies → picked two colonies → i5-10-1</span></p><p class="c2"><span> → i5-10-2</span></p><p class="c2"><span>7/24/2013</span></p><p class="c2"><span>Miniprep</span></p><p class="c2"><span>i5-10-1[GFP] 98.4 ng/ul</span></p><p class="c2"><span>i5-10-2[GFP] 61.4 ng/ul</span></p><p class="c2"><span>i5-10-3[pLac] 46.9 ng/ul</span></p><p class="c2"><span>i5-10-4[pLac] 61.8 ng/ul </span></p><p class="c1"><span></span></p><p class="c2"><span>Transformation </span></p><p class="c2"><span>NEB cells</span></p><p class="c2"><span>i5-6-3 (positive control) → i5-10-1 → 20 + colonies </span></p><p class="c2"><span>3:1 → i5-10-2 [pLac-GFP] → 2 colonies</span></p><p class="c2"><span>0:1 (negative control) → i5-10-3 [pLAc vector] → 0 colonies </span></p><p class="c1"><span></span></p><p class="c2"><span>7/25/2013</span></p><p class="c1"><span></span></p><p class="c2"><span>DNA Isolation </span></p><p class="c2"><span>MGI655 → i5-11-1 34.8 ng/ul</span></p><p class="c2"><span> → i5-11-2 60.7 ng/ul</span></p><p class="c2"><span> → i5-11-3 15.5 ng/ul</span></p><p class="c2"><span> → i5-11-4 35.1 ng/ul</span></p><p class="c1"><span></span></p><p class="c2"><span>Enzymatic lysis buffer:</span></p><p class="c2"><span>20 ul Tris-EDTA</span></p><p class="c2"><span>12 ul Triton </span></p><p class="c2"><span>912 ul Water</span></p><p class="c2"><span>______________</span></p><p class="c2"><span>56 ul lysozyme (add right before use)</span></p><p class="c1"><span></span></p><p class="c2"><span>7/26/2013</span></p><p class="c1"><span></span></p><p class="c2"><span>Glycerol Stock </span></p><p class="c2"><span>i5-10-4 → i5-11-5 </span></p><p class="c2"><span>i5-10-5 → i5-11-6</span></p><p class="c1"><span></span></p><p class="c2"><span>Miniprep </span></p><p class="c2"><span>i5-10-4 → i5-11-5 54.7 ng/ul [pLac-RBS-GFP]</span></p><p class="c2"><span>i5-10-5 → i5-11-6 49.2 ng/ul [pLac-RBS-GFP]</span></p><p class="c1"><span></span></p><p class="c2"><span>PCR Reaction (MGI655)</span></p><p class="c1"><span></span></p><a href="#" name="fb2bbd567864d0e2cdd50e7c82681985e2e636d5"></a><a href="#" name="6"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c29"><p class="c1 c4"><span></span></p></td><td class="c89"><p class="c4 c2"><span>8</span></p></td><td class="c102"><p class="c4 c2"><span>9</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>DNA</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Primer (8F)</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>R 1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Primer (8R)</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>F 1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Polymerase (pFusion)</span></p></td><td class="c89"><p class="c4 c2"><span>10</span></p></td><td class="c102"><p class="c4 c2"><span>10</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>H₂O </span></p></td><td class="c89"><p class="c4 c2"><span>7</span></p></td><td class="c102"><p class="c4 c2"><span>7</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Total </span></p></td><td class="c89"><p class="c4 c2"><span>20</span></p></td><td class="c102"><p class="c4 c2"><span>20</span></p></td></tr></tbody></table><p class="c1"><span></span></p><p class="c2"><span>DNA Isolation with RNeasy Blood and Tissue Kit</span></p><p class="c2"><span>S.Mutans --></span></p><p class="c2"><span>i5-12-1 → 88.3 ng/ul</span></p><p class="c2"><span>i5-12-2 → 30.3 ng/ul </span></p><p class="c1"><span></span></p><p class="c2"><span>7/27/2012</span></p><p class="c1"><a name="h.c74wfbk0ki91"></a></p><a href="#" name="568664dec79d8d575aaf1cec83403fa8c1a3ad50"></a><a href="#" name="7"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.x80mk69q957f"></a><span>Primer</span></p></td><td class="c87"><p class="c4 c2"><a name="h.lbc1tyn3yika"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.kpjc1dskz0r9"></a><span>2</span></p></td><td class="c26"><p class="c4 c2"><a name="h.hw3zrhyk2pdl"></a><span>3</span></p></td><td class="c26"><p class="c4 c2"><a name="h.jxlb5edmq6li"></a><span>4</span></p></td><td class="c26"><p class="c4 c2"><a name="h.det2fdtn1z2t"></a><span>5</span></p></td><td class="c26"><p class="c4 c2"><a name="h.q7u5n4r5fca7"></a><span>6</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vi3hb65hn03k"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.muyiuvqm8zg"></a><span>8</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mm81mm7tll0x"></a><span>9</span></p></td><td class="c26"><p class="c4 c2"><a name="h.kfwgk9phgoud"></a><span>10</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.kasipbw7l7q"></a><span>DNA</span></p></td><td class="c87"><p class="c4 c2"><a name="h.i9dwvpq5noho"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.61vxj34hxkvb"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.1zfxoe8f692d"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.dbeix02xwbep"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ftxj9rii53uz"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.713p8m2nv76l"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ofblf8t9axf8"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.k698esb6eeli"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ml5firq4a49"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.5osr8pdtzuqs"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.cw0pwuwumr28"></a><span>Forward</span></p></td><td class="c87"><p class="c4 c2"><a name="h.zfc42kp5da47"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.ac7j131q4uia"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mrl9catt06o5"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ox5es01oni9k"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.5snlje483gyo"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.kgcuw9be9o5v"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mbrwokdfb7f5"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ju7eg3tn6xp"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vk1we9vrmzyt"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.8aerfpmc3ewm"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.lvt2xtuz7nfx"></a><span>Reverse</span></p></td><td class="c87"><p class="c4 c2"><a name="h.rg728du8xg5"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.71nik2lgl8ex"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.n41bhxtl2mgo"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vbdtmg15wtbf"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.qgdjpccu5y6c"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.npc4cn3yr3es"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c6bwc5h8m4nk"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.sn4clqi8f1n0"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.y6ptei6bjx3f"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.m2nwg1rustsy"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.sq1rw5ffdqcq"></a><span>Phusion </span></p></td><td class="c87"><p class="c4 c2"><a name="h.dczrad3ll1mv"></a><span>10</span></p></td><td class="c43"><p class="c4 c2"><a name="h.kzxwyaz9wz9"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.2vqbu3sc5ab"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.wu32cplrcglv"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.wirojc6d4snp"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.pscsdxa33omg"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.z6vdw2jwwuqx"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.pnt4xdtz05ne"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.123cxn5gbnrc"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.uiwgkugfizlm"></a><span>10</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.ovdj40su5h7d"></a><span>Water</span></p></td><td class="c87"><p class="c4 c2"><a name="h.ttmmg3mlbr01"></a><span>7</span></p></td><td class="c43"><p class="c4 c2"><a name="h.7yuio2gunhyy"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.lfps3rbohioe"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vvbnf0qym3r"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.eu2pv3427mvz"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c0yscubylyim"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ais0f5cxkimo"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ijkk50bc8vfc"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.1z3sb2ooz031"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c9f2uuw3g1k9"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.f9vr6a4tau82"></a></p><p class="c2"><a name="h.2v8293oxl31m"></a><span>7/27/2013</span></p><p class="c1"><a name="h.3yl3n1uiaccl"></a></p><a href="#" name="104372c6427563de636b62fe345d95e734a96dae"></a><a href="#" name="8"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.lif5uh8a9ru5"></a><span>1</span></p></td><td class="c24"><p class="c4 c2"><a name="h.skiksmnn8a6s"></a><span>i5-13-1</span></p></td><td class="c17"><p class="c4 c2"><a name="h.z7jenu3w39sb"></a><span>18.4</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.z0hca4r5b8qn"></a><span>2</span></p></td><td class="c24"><p class="c4 c2"><a name="h.f3nxtwm21n6m"></a><span>i5-13-2</span></p></td><td class="c17"><p class="c4 c2"><a name="h.uqvy6aqkj78d"></a><span>9.7</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.axhxqpai8z1f"></a><span>3</span></p></td><td class="c24"><p class="c4 c2"><a name="h.rhsnpzxmd1h8"></a><span>Failed</span></p></td><td class="c17"><p class="c4 c2"><a name="h.9vgxdsujxymk"></a><span>---</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.e1oongaoz4hm"></a><span>4</span></p></td><td class="c24"><p class="c4 c2"><a name="h.44vk83d784r1"></a><span>i5-13-3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.m7xvhd894v72"></a><span>6.6</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.2l8b57cblu"></a><span>5</span></p></td><td class="c24"><p class="c4 c2"><a name="h.ahsuxtk55dkw"></a><span>i5-13-4</span></p></td><td class="c17"><p class="c4 c2"><a name="h.ncz5e124gpbc"></a><span>3.0</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.od8av1xynej2"></a><span>6</span></p></td><td class="c24"><p class="c4 c2"><a name="h.c96gtq9s71y2"></a><span>i5-13-5</span></p></td><td class="c17"><p class="c4 c2"><a name="h.vxhbmifkq0pu"></a><span>4.7</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.6p6boqiu7y13"></a><span>7</span></p></td><td class="c24"><p class="c4 c2"><a name="h.5ncuhw1ecuin"></a><span>i5-13-6</span></p></td><td class="c17"><p class="c4 c2"><a name="h.wkv6va6u5opu"></a><span>6.2</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.za7l81wf53cb"></a><span>8</span></p></td><td class="c24"><p class="c4 c2"><a name="h.gvxjhuaekgc5"></a><span>Failed</span></p></td><td class="c17"><p class="c4 c2"><a name="h.m2gww3x6td12"></a><span>---</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.ujpyzzvmoq1h"></a><span>9</span></p></td><td class="c24"><p class="c4 c2"><a name="h.tfh4pztzb4ta"></a><span>i5-13-7</span></p></td><td class="c17"><p class="c4 c2"><a name="h.c0czudjusars"></a><span>6.2</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.3z9hssn7h1v1"></a><span>10</span></p></td><td class="c24"><p class="c4 c2"><a name="h.jtgwcyr3oldj"></a><span>i5-13-8</span></p></td><td class="c17"><p class="c4 c2"><a name="h.mavxc2ewd4cr"></a><span>7.3</span></p></td></tr></tbody></table><p class="c1"><a name="h.kdsbkjgtrmox"></a></p><p class="c2"><a name="h.hbpr7r9p3d9"></a><span></span></p> | <p class="c2"><span>7/22/2013</span></p><p class="c2"><span>[GFP] – I3-5-8 Carb Resistance</span></p><p class="c2"><span>[pLac]- I5-6-3 Chlora</span></p><p class="c2"><span>I4-45-2 Chlora</span></p><p class="c1"><span></span></p><p class="c2"><span>7/23/2013</span></p><p class="c2"><span>Transformation Results:</span></p><a href="#" name="561d1c16c2e9d617f036d365af80fddf4eda0c9f"></a><a href="#" name="5"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c0"><p class="c1 c4"><span></span></p></td><td class="c0"><p class="c4 c2"><span># of colonies</span></p></td><td class="c0"><p class="c1 c4"><span></span></p></td></tr><tr class="c8"><td class="c0"><p class="c1 c4"><span></span></p></td><td class="c0"><p class="c4 c2"><span>NEB</span></p></td><td class="c0"><p class="c4 c2"><span>Commercial </span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>I4-45-2</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>0</span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>I5-6-3 [pLac]</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>90+</span></p></td></tr><tr class="c8"><td class="c0"><p class="c4 c2"><span>GFP-I3-5-8</span></p></td><td class="c0"><p class="c4 c2"><span>100+</span></p></td><td class="c0"><p class="c4 c2"><span>Tmtc</span></p></td></tr></tbody></table><p class="c1"><span></span></p><p class="c2"><span>[pLac]-I5-6-3 → [NEB] 100+ colonies → picked two colonies → i5-10-3 </span></p><p class="c2"><span> → i5-10-4</span></p><p class="c2"><span>[GFP]-I3-5-8 → [NEB] 100+ colonies → picked two colonies → i5-10-1</span></p><p class="c2"><span> → i5-10-2</span></p><p class="c2"><span>7/24/2013</span></p><p class="c2"><span>Miniprep</span></p><p class="c2"><span>i5-10-1[GFP] 98.4 ng/ul</span></p><p class="c2"><span>i5-10-2[GFP] 61.4 ng/ul</span></p><p class="c2"><span>i5-10-3[pLac] 46.9 ng/ul</span></p><p class="c2"><span>i5-10-4[pLac] 61.8 ng/ul </span></p><p class="c1"><span></span></p><p class="c2"><span>Transformation </span></p><p class="c2"><span>NEB cells</span></p><p class="c2"><span>i5-6-3 (positive control) → i5-10-1 → 20 + colonies </span></p><p class="c2"><span>3:1 → i5-10-2 [pLac-GFP] → 2 colonies</span></p><p class="c2"><span>0:1 (negative control) → i5-10-3 [pLAc vector] → 0 colonies </span></p><p class="c1"><span></span></p><p class="c2"><span>7/25/2013</span></p><p class="c1"><span></span></p><p class="c2"><span>DNA Isolation </span></p><p class="c2"><span>MGI655 → i5-11-1 34.8 ng/ul</span></p><p class="c2"><span> → i5-11-2 60.7 ng/ul</span></p><p class="c2"><span> → i5-11-3 15.5 ng/ul</span></p><p class="c2"><span> → i5-11-4 35.1 ng/ul</span></p><p class="c1"><span></span></p><p class="c2"><span>Enzymatic lysis buffer:</span></p><p class="c2"><span>20 ul Tris-EDTA</span></p><p class="c2"><span>12 ul Triton </span></p><p class="c2"><span>912 ul Water</span></p><p class="c2"><span>______________</span></p><p class="c2"><span>56 ul lysozyme (add right before use)</span></p><p class="c1"><span></span></p><p class="c2"><span>7/26/2013</span></p><p class="c1"><span></span></p><p class="c2"><span>Glycerol Stock </span></p><p class="c2"><span>i5-10-4 → i5-11-5 </span></p><p class="c2"><span>i5-10-5 → i5-11-6</span></p><p class="c1"><span></span></p><p class="c2"><span>Miniprep </span></p><p class="c2"><span>i5-10-4 → i5-11-5 54.7 ng/ul [pLac-RBS-GFP]</span></p><p class="c2"><span>i5-10-5 → i5-11-6 49.2 ng/ul [pLac-RBS-GFP]</span></p><p class="c1"><span></span></p><p class="c2"><span>PCR Reaction (MGI655)</span></p><p class="c1"><span></span></p><a href="#" name="fb2bbd567864d0e2cdd50e7c82681985e2e636d5"></a><a href="#" name="6"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c29"><p class="c1 c4"><span></span></p></td><td class="c89"><p class="c4 c2"><span>8</span></p></td><td class="c102"><p class="c4 c2"><span>9</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>DNA</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Primer (8F)</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>R 1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Primer (8R)</span></p></td><td class="c89"><p class="c4 c2"><span>1</span></p></td><td class="c102"><p class="c4 c2"><span>F 1</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Polymerase (pFusion)</span></p></td><td class="c89"><p class="c4 c2"><span>10</span></p></td><td class="c102"><p class="c4 c2"><span>10</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>H₂O </span></p></td><td class="c89"><p class="c4 c2"><span>7</span></p></td><td class="c102"><p class="c4 c2"><span>7</span></p></td></tr><tr class="c8"><td class="c29"><p class="c4 c2"><span>Total </span></p></td><td class="c89"><p class="c4 c2"><span>20</span></p></td><td class="c102"><p class="c4 c2"><span>20</span></p></td></tr></tbody></table><p class="c1"><span></span></p><p class="c2"><span>DNA Isolation with RNeasy Blood and Tissue Kit</span></p><p class="c2"><span>S.Mutans --></span></p><p class="c2"><span>i5-12-1 → 88.3 ng/ul</span></p><p class="c2"><span>i5-12-2 → 30.3 ng/ul </span></p><p class="c1"><span></span></p><p class="c2"><span>7/27/2012</span></p><p class="c1"><a name="h.c74wfbk0ki91"></a></p><a href="#" name="568664dec79d8d575aaf1cec83403fa8c1a3ad50"></a><a href="#" name="7"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.x80mk69q957f"></a><span>Primer</span></p></td><td class="c87"><p class="c4 c2"><a name="h.lbc1tyn3yika"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.kpjc1dskz0r9"></a><span>2</span></p></td><td class="c26"><p class="c4 c2"><a name="h.hw3zrhyk2pdl"></a><span>3</span></p></td><td class="c26"><p class="c4 c2"><a name="h.jxlb5edmq6li"></a><span>4</span></p></td><td class="c26"><p class="c4 c2"><a name="h.det2fdtn1z2t"></a><span>5</span></p></td><td class="c26"><p class="c4 c2"><a name="h.q7u5n4r5fca7"></a><span>6</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vi3hb65hn03k"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.muyiuvqm8zg"></a><span>8</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mm81mm7tll0x"></a><span>9</span></p></td><td class="c26"><p class="c4 c2"><a name="h.kfwgk9phgoud"></a><span>10</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.kasipbw7l7q"></a><span>DNA</span></p></td><td class="c87"><p class="c4 c2"><a name="h.i9dwvpq5noho"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.61vxj34hxkvb"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.1zfxoe8f692d"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.dbeix02xwbep"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ftxj9rii53uz"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.713p8m2nv76l"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ofblf8t9axf8"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.k698esb6eeli"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ml5firq4a49"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.5osr8pdtzuqs"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.cw0pwuwumr28"></a><span>Forward</span></p></td><td class="c87"><p class="c4 c2"><a name="h.zfc42kp5da47"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.ac7j131q4uia"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mrl9catt06o5"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ox5es01oni9k"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.5snlje483gyo"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.kgcuw9be9o5v"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.mbrwokdfb7f5"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ju7eg3tn6xp"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vk1we9vrmzyt"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.8aerfpmc3ewm"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.lvt2xtuz7nfx"></a><span>Reverse</span></p></td><td class="c87"><p class="c4 c2"><a name="h.rg728du8xg5"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.71nik2lgl8ex"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.n41bhxtl2mgo"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vbdtmg15wtbf"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.qgdjpccu5y6c"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.npc4cn3yr3es"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c6bwc5h8m4nk"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.sn4clqi8f1n0"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.y6ptei6bjx3f"></a><span>1</span></p></td><td class="c26"><p class="c4 c2"><a name="h.m2nwg1rustsy"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.sq1rw5ffdqcq"></a><span>Phusion </span></p></td><td class="c87"><p class="c4 c2"><a name="h.dczrad3ll1mv"></a><span>10</span></p></td><td class="c43"><p class="c4 c2"><a name="h.kzxwyaz9wz9"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.2vqbu3sc5ab"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.wu32cplrcglv"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.wirojc6d4snp"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.pscsdxa33omg"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.z6vdw2jwwuqx"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.pnt4xdtz05ne"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.123cxn5gbnrc"></a><span>10</span></p></td><td class="c26"><p class="c4 c2"><a name="h.uiwgkugfizlm"></a><span>10</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.ovdj40su5h7d"></a><span>Water</span></p></td><td class="c87"><p class="c4 c2"><a name="h.ttmmg3mlbr01"></a><span>7</span></p></td><td class="c43"><p class="c4 c2"><a name="h.7yuio2gunhyy"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.lfps3rbohioe"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.vvbnf0qym3r"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.eu2pv3427mvz"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c0yscubylyim"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ais0f5cxkimo"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.ijkk50bc8vfc"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.1z3sb2ooz031"></a><span>7</span></p></td><td class="c26"><p class="c4 c2"><a name="h.c9f2uuw3g1k9"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.f9vr6a4tau82"></a></p><p class="c2"><a name="h.2v8293oxl31m"></a><span>7/27/2013</span></p><p class="c1"><a name="h.3yl3n1uiaccl"></a></p><a href="#" name="104372c6427563de636b62fe345d95e734a96dae"></a><a href="#" name="8"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.lif5uh8a9ru5"></a><span>1</span></p></td><td class="c24"><p class="c4 c2"><a name="h.skiksmnn8a6s"></a><span>i5-13-1</span></p></td><td class="c17"><p class="c4 c2"><a name="h.z7jenu3w39sb"></a><span>18.4</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.z0hca4r5b8qn"></a><span>2</span></p></td><td class="c24"><p class="c4 c2"><a name="h.f3nxtwm21n6m"></a><span>i5-13-2</span></p></td><td class="c17"><p class="c4 c2"><a name="h.uqvy6aqkj78d"></a><span>9.7</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.axhxqpai8z1f"></a><span>3</span></p></td><td class="c24"><p class="c4 c2"><a name="h.rhsnpzxmd1h8"></a><span>Failed</span></p></td><td class="c17"><p class="c4 c2"><a name="h.9vgxdsujxymk"></a><span>---</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.e1oongaoz4hm"></a><span>4</span></p></td><td class="c24"><p class="c4 c2"><a name="h.44vk83d784r1"></a><span>i5-13-3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.m7xvhd894v72"></a><span>6.6</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.2l8b57cblu"></a><span>5</span></p></td><td class="c24"><p class="c4 c2"><a name="h.ahsuxtk55dkw"></a><span>i5-13-4</span></p></td><td class="c17"><p class="c4 c2"><a name="h.ncz5e124gpbc"></a><span>3.0</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.od8av1xynej2"></a><span>6</span></p></td><td class="c24"><p class="c4 c2"><a name="h.c96gtq9s71y2"></a><span>i5-13-5</span></p></td><td class="c17"><p class="c4 c2"><a name="h.vxhbmifkq0pu"></a><span>4.7</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.6p6boqiu7y13"></a><span>7</span></p></td><td class="c24"><p class="c4 c2"><a name="h.5ncuhw1ecuin"></a><span>i5-13-6</span></p></td><td class="c17"><p class="c4 c2"><a name="h.wkv6va6u5opu"></a><span>6.2</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.za7l81wf53cb"></a><span>8</span></p></td><td class="c24"><p class="c4 c2"><a name="h.gvxjhuaekgc5"></a><span>Failed</span></p></td><td class="c17"><p class="c4 c2"><a name="h.m2gww3x6td12"></a><span>---</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.ujpyzzvmoq1h"></a><span>9</span></p></td><td class="c24"><p class="c4 c2"><a name="h.tfh4pztzb4ta"></a><span>i5-13-7</span></p></td><td class="c17"><p class="c4 c2"><a name="h.c0czudjusars"></a><span>6.2</span></p></td></tr><tr class="c8"><td class="c30"><p class="c4 c2"><a name="h.3z9hssn7h1v1"></a><span>10</span></p></td><td class="c24"><p class="c4 c2"><a name="h.jtgwcyr3oldj"></a><span>i5-13-8</span></p></td><td class="c17"><p class="c4 c2"><a name="h.mavxc2ewd4cr"></a><span>7.3</span></p></td></tr></tbody></table><p class="c1"><a name="h.kdsbkjgtrmox"></a></p><p class="c2"><a name="h.hbpr7r9p3d9"></a><span></span></p> | ||
| + | <p class="c2"><span>7/28/2013</span></p><p class="c2"><a name="h.1w7xzdr4u63c"></a><span>PCR</span></p><p class="c1"><a name="h.qqxu5pia3myb"></a></p><a href="#" name="0069c024bb369e1d214ad9b0518ab8474de052f6"></a><a href="#" name="9"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.l98dou3iv8o"></a><span>Primers</span></p></td><td class="c36"><p class="c4 c2"><a name="h.iylmgzh0plu0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h7cygypzygvd"></a><span>2</span></p></td><td class="c36"><p class="c4 c2"><a name="h.htgvzlr3zeg1"></a><span>3</span></p></td><td class="c36"><p class="c4 c2"><a name="h.c9zuyf785t1k"></a><span>4</span></p></td><td class="c36"><p class="c4 c2"><a name="h.atfwyo73js4d"></a><span>5</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h7dqlwqgya0e"></a><span>6</span></p></td><td class="c36"><p class="c4 c2"><a name="h.6ekecfdp08zm"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.emgt12bv0g4g"></a><span>8</span></p></td><td class="c36"><p class="c4 c2"><a name="h.cmpkt9yv0iat"></a><span>9</span></p></td><td class="c36"><p class="c4 c2"><a name="h.je7t7q6tv1rf"></a><span>10</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.s8aq2lbk47y3"></a><span>DNA</span></p></td><td class="c36"><p class="c4 c2"><a name="h.rwp11tgj4ahh"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.z2tat31a3gar"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.i98vrbkqsqnm"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.usa99qhtlxql"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.yk37pz4wnolg"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.93mvjlyrb5jn"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.dz2ctq9w2aqk"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.sotzy92rv1s"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.li3a7e2bdvn1"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.t6ryvfn516e3"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.e6r7lvtrxib4"></a><span>F</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9c8jtdfzcapw"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.glq9z4l3ebu"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.cy5bp9k13ic0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9d7e08bmzv1d"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.gduu3qbma75b"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.z6t16hk7if5r"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.39dyx564wgqh"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.40oje46pms7d"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.np1vcv5u8g5k"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.emqtdc1e10t6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.azwizg70184b"></a><span>R</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o5hmje4g5c8u"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ukf167shdgjo"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o41h7oavaiam"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.tf8kg14l1y3r"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.irnniwmsayy0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ygpgl6h0jhre"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.i71phhtxr5lq"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o1o13ry40x9f"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.752xc4awlv9t"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.s82d9wj9hh0f"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.wwgb8rb2caqx"></a><span>Phusion</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ot6w07vgsmho"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.j0qp0j8yylz"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9ka3k0y4zl04"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.tf6hvlrn1u79"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.pnybwmk1amlp"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.vyeifkgxpagy"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.vy8zdbxwo69v"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.13lc0p7rp4g"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9rvgw4inrhql"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.bgvpr1ym61fz"></a><span>10</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.zfb5f4i9rdii"></a><span>Water</span></p></td><td class="c36"><p class="c4 c2"><a name="h.sta2t5jqzrvw"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.icutzgtuflsq"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.dvu38r3f8g1t"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.62umehxctlvk"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.1zld32o1mbx4"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.q7dclgac2akm"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.e53dqcg8lwdh"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.thms7q28qo7y"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.5hwyopy6g198"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h268jsv0xfi7"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.ptzum1ut187j"></a></p><p class="c2"><a name="h.625habuoaedf"></a><span>Digestion </span></p><p class="c1"><a name="h.aksdanvknykf"></a></p><a href="#" name="c5f20c3bfc7fb1e9388cd5cdff6e1ead54d8a697"></a><a href="#" name="10"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c51"><p class="c1 c4"><a name="h.537gplby40eo"></a></p></td><td class="c104"><p class="c4 c2"><a name="h.xqc0lppe6krv"></a><span>5 (i5-13-9)</span></p></td><td class="c78"><p class="c4 c2"><a name="h.ubh03cwp1deq"></a><span>6 (i3-13-10)</span></p></td><td class="c76"><p class="c4 c2"><a name="h.o2wbmaardiau"></a><span>7 (i3-13-11)</span></p></td><td class="c33"><p class="c4 c2"><a name="h.ovwu3kvq8nyk"></a><span>8 (i3-13-12)</span></p></td><td class="c96"><p class="c4 c2"><a name="h.y6s4vn5x3iav"></a><span>9 (i3-13-13)</span></p></td><td class="c92"><p class="c4 c2"><a name="h.smdjo587yn7d"></a><span>i5-10-3 (i5-13-14)</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.xntrywweu8of"></a><span>DNA</span></p></td><td class="c104"><p class="c4 c2"><a name="h.lv3064b3lmj"></a><span>30</span></p></td><td class="c78"><p class="c4 c2"><a name="h.8ph34t10kt1k"></a><span>30</span></p></td><td class="c76"><p class="c4 c2"><a name="h.rsoug65yaof9"></a><span>30</span></p></td><td class="c33"><p class="c4 c2"><a name="h.er67n63cg2v7"></a><span>30</span></p></td><td class="c96"><p class="c4 c2"><a name="h.bqbab170861d"></a><span>30</span></p></td><td class="c92"><p class="c4 c2"><a name="h.igemoh6tuopz"></a><span>30</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.3z2okkavkzgn"></a><span>NE Buffer</span></p></td><td class="c104"><p class="c4 c2"><a name="h.23pmrd3j3kfz"></a><span>5</span></p></td><td class="c78"><p class="c4 c2"><a name="h.vsrsr7pdqtt0"></a><span>5</span></p></td><td class="c76"><p class="c4 c2"><a name="h.epa3h1153om3"></a><span>5</span></p></td><td class="c33"><p class="c4 c2"><a name="h.4ikn46vluxyw"></a><span>5</span></p></td><td class="c96"><p class="c4 c2"><a name="h.gxe844eltiua"></a><span>5</span></p></td><td class="c92"><p class="c4 c2"><a name="h.iaz8nua1eyfu"></a><span>5</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.2uuz4e40isx1"></a><span>EcoRI</span></p></td><td class="c104"><p class="c4 c2"><a name="h.i0xp596p5fw1"></a><span>1</span></p></td><td class="c78"><p class="c4 c2"><a name="h.sqlcqmgvj173"></a><span>1</span></p></td><td class="c76"><p class="c4 c2"><a name="h.cq48ztpsv71d"></a><span>1</span></p></td><td class="c33"><p class="c4 c2"><a name="h.ck15m3arqdrd"></a><span>1</span></p></td><td class="c96"><p class="c4 c2"><a name="h.93tkn3hqbi9w"></a><span>1</span></p></td><td class="c92"><p class="c4 c2"><a name="h.w0glvf9ljf32"></a><span>1</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.77qgyz3qlwhs"></a><span>Prst I</span></p></td><td class="c104"><p class="c4 c2"><a name="h.dotv3dly1w9n"></a><span>1</span></p></td><td class="c78"><p class="c4 c2"><a name="h.m0cauz10idxo"></a><span>1</span></p></td><td class="c76"><p class="c4 c2"><a name="h.pzaip3q8vyky"></a><span>1</span></p></td><td class="c33"><p class="c4 c2"><a name="h.5ccfp79x0sya"></a><span>1</span></p></td><td class="c96"><p class="c4 c2"><a name="h.p1vfow1jids1"></a><span>1</span></p></td><td class="c92"><p class="c4 c2"><a name="h.mq5gkij39x1m"></a><span>1</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.tofmw9c4te4v"></a><span>Water</span></p></td><td class="c104"><p class="c4 c2"><a name="h.6dwq0bt714dx"></a><span>13</span></p></td><td class="c78"><p class="c4 c2"><a name="h.t6qsxr11k62i"></a><span>13</span></p></td><td class="c76"><p class="c4 c2"><a name="h.dbq1oskgtj8a"></a><span>13</span></p></td><td class="c33"><p class="c4 c2"><a name="h.j6u49lawj21h"></a><span>13</span></p></td><td class="c96"><p class="c4 c2"><a name="h.loge284qwxle"></a><span>13</span></p></td><td class="c92"><p class="c4 c2"><a name="h.vmjjkyo4l4vt"></a><span>13</span></p></td></tr></tbody></table><p class="c2"><a name="h.kvb5pahdagn6"></a><span>7/24/2013</span></p><p class="c2"><a name="h.d8tsh5kkz4h0"></a><span>PCR</span></p><p class="c1"><a name="h.hskqvv6hw324"></a></p><a href="#" name="a83db890a01d761808d0df4a6f54456aee0869c6"></a><a href="#" name="11"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.q47n76xr18ri"></a><span>Primers</span></p></td><td class="c3"><p class="c4 c2"><a name="h.7rkpqmb3uwac"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.8kvhh4ls95g1"></a><span>2</span></p></td><td class="c3"><p class="c4 c2"><a name="h.l6ktdui81p2i"></a><span>3</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wg2w0swmphbf"></a><span>4</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.aupmsss8kqaz"></a><span>DNA</span></p></td><td class="c3"><p class="c4 c2"><a name="h.us5i9ie2ief7"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.d1arncc0oq0i"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wx2lt1gmgejp"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.moqlznfx8kxv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.ea6xpevky1n5"></a><span>R</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2lo40l8nn351"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.c4nvgln2gort"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.l4ns7ijsrf9a"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.v67x0t1gynjr"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.g728ofbrsyd2"></a><span>F</span></p></td><td class="c3"><p class="c4 c2"><a name="h.log4kdfc2xut"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2tilhu3yk376"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.mrnv1jd0m6vk"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.19fqyxi4lj5b"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.wgb3khr5skw2"></a><span>Phusion</span></p></td><td class="c3"><p class="c4 c2"><a name="h.63er7ixlb9ud"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.3mxustbm97sx"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.katv3qd44lkw"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.q7gjuhvs0um"></a><span>10</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.4hdp70vtlra"></a><span>Water</span></p></td><td class="c3"><p class="c4 c2"><a name="h.sndqj4z3vcvl"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.1f065lbzz1z"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.gvon0ul75w87"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.xoffqy49dsnd"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.vw3bshxwwuxf"></a></p><p class="c2"><a name="h.koawdqsmnyoh"></a><span>1 i5-14-1 33.3 ng/ul (Com C)</span></p><p class="c2"><a name="h.fv1vt78mg7ru"></a><span>2 i5-14-2 47.5 ng/ul (Com D)</span></p><p class="c2"><a name="h.3x1x0benh4pe"></a><span>3 i5-14-3 38.4 ng/ul (Com E)</span></p><p class="c2"><a name="h.4z3kd4o6avp1"></a><span>4 i5-14-4 27.8 ng/ul (Com A)</span></p><p class="c1"><a name="h.l08jj5jvxhhm"></a></p><p class="c2"><a name="h.byev1ekaa63a"></a><span>7/30/2013</span></p><p class="c2"><a name="h.hhq1lr5p0okp"></a><span>Digestion </span></p><p class="c2"><a name="h.rz8lt1ph5jxn"></a><span>1 (i5-14-1) (Com C)</span></p><p class="c2"><a name="h.n0uh9rz1mf4q"></a><span>DNA 30 (0 left)</span></p><p class="c2"><a name="h.kneof781dyz1"></a><span>NEBuffer 5</span></p><p class="c2"><a name="h.i4qtg9crqsm6"></a><span>EcoRI 1</span></p><p class="c2"><a name="h.db59aiebk0lj"></a><span>PstI 1</span></p><p class="c2"><a name="h.fw9odm6ololp"></a><span>Water 13</span></p><p class="c2"><a name="h.ut30mxfqsmhf"></a><span>→ new i5-14-1</span></p><p class="c1"><a name="h.2flutjvnrfl1"></a></p><p class="c2"><a name="h.qyltrisqlr82"></a><span>Gel extraction i5-14-1</span></p><p class="c1"><a name="h.lag2kt5dnjgh"></a></p><p class="c2"><a name="h.mvk988hr22kv"></a><span>i5-13-9 (Com B) 8.6 ng/ul </span></p><p class="c2"><a name="h.dj3ugc0biey"></a><span>i5-13-10 (ScrR) 12 ng/ul</span></p><p class="c2"><a name="h.5qit7p46px2g"></a><span>i513-11(pScr) 14.4 ng/ul</span></p><p class="c2"><a name="h.3do6oe7lvk2u"></a><span>i5-13-12 (Cra) 15.1 ng/ul</span></p><p class="c2"><a name="h.5y516xtktq34"></a><span>i5-13-13(pFru) 11 ng/ul</span></p><p class="c2"><a name="h.4boqh7b34zdl"></a><span>i5-13-14(Chlora backbone) 24 ng/ul</span></p><p class="c2"><a name="h.s5lp4mbltzhy"></a><span>i5-15-1 (Com C) 13.9 ng/ul</span></p><p class="c1"><a name="h.8r9vz5eadw0t"></a></p><p class="c2"><a name="h.ut6ozfvulmxz"></a><span>Ligation </span></p><p class="c2"><a name="h.pfehlplbqkke"></a><span>Vector i5-13-14 (Chlora)</span></p><p class="c1"><a name="h.8r7mn68yco2x"></a></p><p class="c1"><a name="h.i07o62sb0skt"></a></p><a href="#" name="50d13a574052472cd642da77fe9561faf1a141d9"></a><a href="#" name="12"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.rwclbd7ju5o6"></a><span>Insert:</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eapxxn7uc1g6"></a><span>i5-13-9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.8yh49v9ynpyf"></a><span>i5-13-9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.z9qy3zix2fy9"></a><span>i5-13-10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.pjoqdlvv3lkm"></a><span>i5-13-10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wq1gnmk3rbzf"></a><span>i5-13-11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n8s28blj6ch8"></a><span>i5-13-11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.vzyxdfb5t3sd"></a><span>i5-13-12</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2qrdexvawwpk"></a><span>i5-13-12</span></p></td><td class="c18"><p class="c4 c2"><a name="h.z6v5lw64ahad"></a><span>i5-13-13</span></p></td><td class="c18"><p class="c4 c2"><a name="h.y5i9fqvu88i6"></a><span>i5-13-13</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ucy77na90940"></a><span>i5-15-1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n4drv21h8wzz"></a><span>i5-15-1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c1 c4"><a name="h.ddozs2852nhp"></a></p></td><td class="c18"><p class="c4 c2"><a name="h.4v3arya9n04w"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.lze3kxcsc4ks"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7gpyhvx1ddwn"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.tb20iaswej"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.hf9fz1ks5wzs"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.yvfgmo49urdc"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xbpyfe5ghwhd"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.s365vqt413uu"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.tj1zd9fyq725"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gv8xkigvkbxs"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bn78bbm9lzz5"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.11iltw2g7gja"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.8xwue3hhxoj2"></a><span>Vector</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ueapcumgr70z"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.a90678jyza68"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e6e899jjwqh"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.v9uy9dru9lha"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7s651ehvei4b"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.i107go106z3z"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xhtik8jfr5t8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7jycjrl59rkh"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bw4ew4c4843j"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.1yf4govsdmmz"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.dy0o6cu8osv8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e1bg38dvm6ts"></a><span>2</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.cykgsr2weglr"></a><span>insert</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eizukidl4cyc"></a><span>7.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.kog90q6bx275"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.98vnva3vgr2u"></a><span>6</span></p></td><td class="c18"><p class="c4 c2"><a name="h.9u7gxwmfqfpt"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.azeiwoyo9gg3"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.shdnosdzcw16"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xhdyltdzx6ea"></a><span>5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.qb978w6jidz"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.mdvwwm8w3gr2"></a><span>1.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.berxsw3ctoj9"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bws8wghda8eq"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.exx4y5x68751"></a><span>0</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.ufw4a7mv08qf"></a><span>buffer</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e82ys14lie5g"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.w3awm2jc8x62"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ip79buro7z35"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2jwvv8pad0y8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eui2glumbma9"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ij6a4wggvhr8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.5erup0qxiaoa"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.34drqu9lsiip"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sxt14q53zna"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zeib64cg79d2"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.w76ejfa0vgam"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sli6mh394kb5"></a><span>2</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.v35ueq5cr2ki"></a><span>ligase</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jp2k3gq27m9p"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l2ezm01epi51"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ep4rxobcrnm0"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gr1lj3ltfo95"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gf8vy915vn"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.trcwr9gqzc95"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ap4irlbntl3b"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.brtf4pa8s3tg"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.56a01qf31wqd"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.8nysqqn9xpga"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.cgwytf5eesu9"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.b39j5p6tch1k"></a><span>1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.kf7kmjciwkpj"></a><span>water</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l5mxf8eibg1"></a><span>7.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.4hvglti0oxpi"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jr1bst19a9wz"></a><span>9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wzkvjibfhcnb"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.pndyfze6qn5i"></a><span>14</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gub9i3ohcehi"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.i2kexdv8pq33"></a><span>10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.o7nzrkza94cd"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xpe8r41l59s0"></a><span>13.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.55gekl6q1m72"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ym8dfmbzpnfk"></a><span>14</span></p></td><td class="c18"><p class="c4 c2"><a name="h.1nuuijugz687"></a><span>15</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.xt2svhv3b8l4"></a><span>total</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2tn6w1uqmqxz"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sdpcuzjyl7ts"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ourvjjokdadc"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bsdbvy1o7wln"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jwaa3xuxas9"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.hqmcbdgpw0lx"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e0ehwrwd4agz"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zfiuqf321edu"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ps0ewmqtqv9o"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n6pyhtm59dbi"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.y4lt0enlcu3u"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.riegvhuvgl4n"></a><span>20</span></p></td></tr><tr class="c8"><td class="c18"><p class="c1 c4"><a name="h.abfr1wtzld8l"></a></p></td><td class="c18"><p class="c4 c2"><a name="h.eue2n4kohh0l"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ef50nw1y7qha"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2l6f7excv42h"></a><span>3</span></p></td><td class="c18"><p class="c4 c2"><a name="h.3c6fmyyunrm7"></a><span>4</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l5ht1kgx2r3d"></a><span>5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zi9axt96eqt2"></a><span>6</span></p></td><td class="c18"><p class="c4 c2"><a name="h.qr6e4yv2ily7"></a><span>7</span></p></td><td class="c18"><p class="c4 c2"><a name="h.exxb2fxp0zbw"></a><span>8</span></p></td><td class="c18"><p class="c4 c2"><a name="h.kuz4x1yluh03"></a><span>9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7luzyy8w7tqz"></a><span>10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wzwxx8s2jhfe"></a><span>11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.c4dyb9ecvegy"></a><span>12</span></p></td></tr></tbody></table><p class="c1"><a name="h.gfculfarh7ou"></a></p><p class="c2"><a name="h.mhtosyfxiplo"></a><span>Transformation </span></p><p class="c2"><a name="h.i3wtjhdwzin3"></a><span>1</span></p><p class="c2"><a name="h.yqiuna5qzuv"></a><span>2</span></p><p class="c2"><a name="h.6506ohlh2qwn"></a><span>3</span></p><p class="c2"><a name="h.ic0zbvoiwg54"></a><span>4</span></p><p class="c2"><a name="h.7jw4qtpuq8u"></a><span>5</span></p><p class="c2"><a name="h.vxoc92u740ec"></a><span>6</span></p><p class="c2"><a name="h.xw9bu3ftnlrz"></a><span>7</span></p><p class="c2"><a name="h.wz32he1cowmw"></a><span>8</span></p><p class="c2"><a name="h.xu12aug1fjsk"></a><span>9</span></p><p class="c2"><a name="h.6nxy36gth9ap"></a><span>10</span></p><p class="c2"><a name="h.6vj90p5kdqyh"></a><span>11</span></p><p class="c2"><a name="h.2ivfpv951rq5"></a><span>12</span></p><p class="c2"><a name="h.xrfzapxgozv0"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.a30bxd2gr253"></a></p><p class="c2"><a name="h.2sye30drygjv"></a><span>Inconsistent growth (repeat)</span></p><p class="c1"><a name="h.rcwo2mwhmsj8"></a></p><p class="c2"><a name="h.qdwpmlar9fxp"></a><span>7/30</span></p><p class="c2"><a name="h.h2yrqpht6uek"></a><span>Transformation</span></p><p class="c2"><a name="h.g16bsupb0if7"></a><span>1</span></p><p class="c2"><a name="h.yepbrmq46j8j"></a><span>2</span></p><p class="c2"><a name="h.yuzf97e1ycl1"></a><span>3</span></p><p class="c2"><a name="h.4k2gx4fcrj7c"></a><span>4</span></p><p class="c2"><a name="h.ytz71xaxz9i"></a><span>5</span></p><p class="c2"><a name="h.y9wrgq1sinlr"></a><span>6</span></p><p class="c2"><a name="h.f4nyxt67c1g6"></a><span>7</span></p><p class="c2"><a name="h.chgf8ey2p72w"></a><span>8</span></p><p class="c2"><a name="h.6yor6rdyrcfh"></a><span>9</span></p><p class="c2"><a name="h.ysyhpy20dxld"></a><span>10</span></p><p class="c2"><a name="h.wgqbqis3fu9k"></a><span>11</span></p><p class="c2"><a name="h.g2va1il9rme3"></a><span>12</span></p><p class="c2"><a name="h.va67wmeyjr7z"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.l3gyqob9ad8r"></a></p><p class="c2"><a name="h.lyf956klvtnq"></a><span>Inoculate</span></p><p class="c2"><a name="h.cu4kdzhfgn9j"></a><span>From glycerol stock i5-11-5 (pLac-GFP)</span></p><p class="c2"><a name="h.cvxy2odcnx2"></a><span>100.4 ng/ul</span></p><p class="c2"><a name="h.72910tlji9sm"></a><span>96.4 ng/ul [pLac-GFP]</span></p><p class="c2"><a name="h.gxyw4fotd64k"></a><span>84.3 ng/ul</span></p><p class="c1"><a name="h.blxr8wovn17y"></a></p><p class="c2"><a name="h.1sjg3n4ze7nq"></a><span>7/31</span></p><p class="c2"><a name="h.iwchhdbervw9"></a><span>Transformation Results</span></p><p class="c2"><a name="h.5atic82knicq"></a><span>1 [Com B] → 8 colonies → Picked 3 colonies→ i5-16-1</span></p><p class="c2"><a name="h.k4hslldhwd8r"></a><span> i5-16-2</span></p><p class="c2"><a name="h.16gg4u89efie"></a><span> i5-16-3</span></p><p class="c2"><a name="h.9m4le8otw8qu"></a><span>2→ 50 colonies</span></p><p class="c2"><a name="h.tbck2neavtxc"></a><span>3 [Scr] → 11 colonies → Picked 3 colonies → i5-16-4</span></p><p class="c2"><a name="h.orwfvyt5ue2v"></a><span> i5-16-5</span></p><p class="c2"><a name="h.242xprdzg0b5"></a><span> i5-16-6</span></p><p class="c2"><a name="h.8faqzlhxo0iq"></a><span>4 → 20 colonies</span></p><p class="c2"><a name="h.ds7rd5ef53tw"></a><span>5 [pScr] → 25 colonies → picked 3 colonies → i5-16-7</span></p><p class="c2"><a name="h.uninw76k2wf"></a><span> i5-16-8</span></p><p class="c2"><a name="h.qpdj9aujpd2t"></a><span> i5-16-9</span></p><p class="c2"><a name="h.405vih9kuvu"></a><span>6 → 0 colonies</span></p><p class="c2"><a name="h.ox6mwhqz0f0f"></a><span>7 [Cra] → 50+ colonies → picked 3 colonies → i5-16-10</span></p><p class="c2"><a name="h.3pqoz5468f0a"></a><span> i5-16-11</span></p><p class="c2"><a name="h.g56vdta42xvu"></a><span> i5-16-12</span></p><p class="c2"><a name="h.uf67ey9w9f40"></a><span>8 → 75+ colonies</span></p><p class="c2"><a name="h.aoggldrolu7c"></a><span>9 [pFru] → 75+ colonies → picked 3 colonies → i5-16-13</span></p><p class="c2"><a name="h.z6au5v2jb975"></a><span> i5-16-14</span></p><p class="c2"><a name="h.90uwnto7k70s"></a><span> i5-16-15</span></p><p class="c2"><a name="h.n89z936984bi"></a><span>10 → 11 colonies</span></p><p class="c2"><a name="h.283avhppju1z"></a><span>11 [Com C] → 30 colonies → picked 3 colonies→ i5-16-16</span></p><p class="c2"><a name="h.92qdy4j90bwo"></a><span> i5-16-17</span></p><p class="c2"><a name="h.wsv606ce7ery"></a><span> i5-16-18</span></p><p class="c2"><a name="h.ubmjrqntcnja"></a><span>12→ 30 colonies</span></p><p class="c2"><a name="h.mj4433clon1w"></a><span>13→ 7 colonies</span></p><p class="c2"><a name="h.u0ea7xjc6274"></a><span>14 →2 colonies</span></p><p class="c2"><a name="h.d9vx3nyuimz7"></a><span>15 → 20 colonies</span></p><p class="c2"><a name="h.f2cq7ihd00rt"></a><span>16→ 10 colonies</span></p><p class="c2"><a name="h.9cfq1743wgqc"></a><span>17 → 0 colonies </span></p><p class="c2"><a name="h.okzp12kpgfhd"></a><span>18 → 5 colonies</span></p><p class="c2"><a name="h.m6j8vq7xdy0l"></a><span>+ control → ∞ colonies</span></p> | ||
Latest revision as of 21:01, 23 September 2013
7/22/2013
[GFP] – I3-5-8 Carb Resistance
[pLac]- I5-6-3 Chlora
I4-45-2 Chlora
7/23/2013
Transformation Results:
<a href="#" name="561d1c16c2e9d617f036d365af80fddf4eda0c9f"></a><a href="#" name="5"></a># of colonies | ||
NEB | Commercial | |
I4-45-2 | 100+ | 0 |
I5-6-3 [pLac] | 100+ | 90+ |
GFP-I3-5-8 | 100+ | Tmtc |
[pLac]-I5-6-3 → [NEB] 100+ colonies → picked two colonies → i5-10-3
→ i5-10-4
[GFP]-I3-5-8 → [NEB] 100+ colonies → picked two colonies → i5-10-1
→ i5-10-2
7/24/2013
Miniprep
i5-10-1[GFP] 98.4 ng/ul
i5-10-2[GFP] 61.4 ng/ul
i5-10-3[pLac] 46.9 ng/ul
i5-10-4[pLac] 61.8 ng/ul
Transformation
NEB cells
i5-6-3 (positive control) → i5-10-1 → 20 + colonies
3:1 → i5-10-2 [pLac-GFP] → 2 colonies
0:1 (negative control) → i5-10-3 [pLAc vector] → 0 colonies
7/25/2013
DNA Isolation
MGI655 → i5-11-1 34.8 ng/ul
→ i5-11-2 60.7 ng/ul
→ i5-11-3 15.5 ng/ul
→ i5-11-4 35.1 ng/ul
Enzymatic lysis buffer:
20 ul Tris-EDTA
12 ul Triton
912 ul Water
______________
56 ul lysozyme (add right before use)
7/26/2013
Glycerol Stock
i5-10-4 → i5-11-5
i5-10-5 → i5-11-6
Miniprep
i5-10-4 → i5-11-5 54.7 ng/ul [pLac-RBS-GFP]
i5-10-5 → i5-11-6 49.2 ng/ul [pLac-RBS-GFP]
PCR Reaction (MGI655)
<a href="#" name="fb2bbd567864d0e2cdd50e7c82681985e2e636d5"></a><a href="#" name="6"></a>
8 | 9 | |
DNA | 1 | 1 |
Primer (8F) | 1 | R 1 |
Primer (8R) | 1 | F 1 |
Polymerase (pFusion) | 10 | 10 |
H₂O | 7 | 7 |
Total | 20 | 20 |
DNA Isolation with RNeasy Blood and Tissue Kit
S.Mutans -->
i5-12-1 → 88.3 ng/ul
i5-12-2 → 30.3 ng/ul
7/27/2012
<a name="h.c74wfbk0ki91"></a>
<a href="#" name="568664dec79d8d575aaf1cec83403fa8c1a3ad50"></a><a href="#" name="7"></a><a name="h.x80mk69q957f"></a>Primer | <a name="h.lbc1tyn3yika"></a>1 | <a name="h.kpjc1dskz0r9"></a>2 | <a name="h.hw3zrhyk2pdl"></a>3 | <a name="h.jxlb5edmq6li"></a>4 | <a name="h.det2fdtn1z2t"></a>5 | <a name="h.q7u5n4r5fca7"></a>6 | <a name="h.vi3hb65hn03k"></a>7 | <a name="h.muyiuvqm8zg"></a>8 | <a name="h.mm81mm7tll0x"></a>9 | <a name="h.kfwgk9phgoud"></a>10 |
<a name="h.kasipbw7l7q"></a>DNA | <a name="h.i9dwvpq5noho"></a>1 | <a name="h.61vxj34hxkvb"></a>1 | <a name="h.1zfxoe8f692d"></a>1 | <a name="h.dbeix02xwbep"></a>1 | <a name="h.ftxj9rii53uz"></a>1 | <a name="h.713p8m2nv76l"></a>1 | <a name="h.ofblf8t9axf8"></a>1 | <a name="h.k698esb6eeli"></a>1 | <a name="h.ml5firq4a49"></a>1 | <a name="h.5osr8pdtzuqs"></a>1 |
<a name="h.cw0pwuwumr28"></a>Forward | <a name="h.zfc42kp5da47"></a>1 | <a name="h.ac7j131q4uia"></a>1 | <a name="h.mrl9catt06o5"></a>1 | <a name="h.ox5es01oni9k"></a>1 | <a name="h.5snlje483gyo"></a>1 | <a name="h.kgcuw9be9o5v"></a>1 | <a name="h.mbrwokdfb7f5"></a>1 | <a name="h.ju7eg3tn6xp"></a>1 | <a name="h.vk1we9vrmzyt"></a>1 | <a name="h.8aerfpmc3ewm"></a>1 |
<a name="h.lvt2xtuz7nfx"></a>Reverse | <a name="h.rg728du8xg5"></a>1 | <a name="h.71nik2lgl8ex"></a>1 | <a name="h.n41bhxtl2mgo"></a>1 | <a name="h.vbdtmg15wtbf"></a>1 | <a name="h.qgdjpccu5y6c"></a>1 | <a name="h.npc4cn3yr3es"></a>1 | <a name="h.c6bwc5h8m4nk"></a>1 | <a name="h.sn4clqi8f1n0"></a>1 | <a name="h.y6ptei6bjx3f"></a>1 | <a name="h.m2nwg1rustsy"></a>1 |
<a name="h.sq1rw5ffdqcq"></a>Phusion | <a name="h.dczrad3ll1mv"></a>10 | <a name="h.kzxwyaz9wz9"></a>10 | <a name="h.2vqbu3sc5ab"></a>10 | <a name="h.wu32cplrcglv"></a>10 | <a name="h.wirojc6d4snp"></a>10 | <a name="h.pscsdxa33omg"></a>10 | <a name="h.z6vdw2jwwuqx"></a>10 | <a name="h.pnt4xdtz05ne"></a>10 | <a name="h.123cxn5gbnrc"></a>10 | <a name="h.uiwgkugfizlm"></a>10 |
<a name="h.ovdj40su5h7d"></a>Water | <a name="h.ttmmg3mlbr01"></a>7 | <a name="h.7yuio2gunhyy"></a>7 | <a name="h.lfps3rbohioe"></a>7 | <a name="h.vvbnf0qym3r"></a>7 | <a name="h.eu2pv3427mvz"></a>7 | <a name="h.c0yscubylyim"></a>7 | <a name="h.ais0f5cxkimo"></a>7 | <a name="h.ijkk50bc8vfc"></a>7 | <a name="h.1z3sb2ooz031"></a>7 | <a name="h.c9f2uuw3g1k9"></a>7 |
<a name="h.f9vr6a4tau82"></a>
<a name="h.2v8293oxl31m"></a>7/27/2013
<a name="h.3yl3n1uiaccl"></a>
<a href="#" name="104372c6427563de636b62fe345d95e734a96dae"></a><a href="#" name="8"></a><a name="h.lif5uh8a9ru5"></a>1 | <a name="h.skiksmnn8a6s"></a>i5-13-1 | <a name="h.z7jenu3w39sb"></a>18.4 |
<a name="h.z0hca4r5b8qn"></a>2 | <a name="h.f3nxtwm21n6m"></a>i5-13-2 | <a name="h.uqvy6aqkj78d"></a>9.7 |
<a name="h.axhxqpai8z1f"></a>3 | <a name="h.rhsnpzxmd1h8"></a>Failed | <a name="h.9vgxdsujxymk"></a>--- |
<a name="h.e1oongaoz4hm"></a>4 | <a name="h.44vk83d784r1"></a>i5-13-3 | <a name="h.m7xvhd894v72"></a>6.6 |
<a name="h.2l8b57cblu"></a>5 | <a name="h.ahsuxtk55dkw"></a>i5-13-4 | <a name="h.ncz5e124gpbc"></a>3.0 |
<a name="h.od8av1xynej2"></a>6 | <a name="h.c96gtq9s71y2"></a>i5-13-5 | <a name="h.vxhbmifkq0pu"></a>4.7 |
<a name="h.6p6boqiu7y13"></a>7 | <a name="h.5ncuhw1ecuin"></a>i5-13-6 | <a name="h.wkv6va6u5opu"></a>6.2 |
<a name="h.za7l81wf53cb"></a>8 | <a name="h.gvxjhuaekgc5"></a>Failed | <a name="h.m2gww3x6td12"></a>--- |
<a name="h.ujpyzzvmoq1h"></a>9 | <a name="h.tfh4pztzb4ta"></a>i5-13-7 | <a name="h.c0czudjusars"></a>6.2 |
<a name="h.3z9hssn7h1v1"></a>10 | <a name="h.jtgwcyr3oldj"></a>i5-13-8 | <a name="h.mavxc2ewd4cr"></a>7.3 |
<a name="h.kdsbkjgtrmox"></a>
<a name="h.hbpr7r9p3d9"></a>
7/28/2013
<a name="h.1w7xzdr4u63c"></a>PCR
<a name="h.qqxu5pia3myb"></a>
<a href="#" name="0069c024bb369e1d214ad9b0518ab8474de052f6"></a><a href="#" name="9"></a><a name="h.l98dou3iv8o"></a>Primers | <a name="h.iylmgzh0plu0"></a>1 | <a name="h.h7cygypzygvd"></a>2 | <a name="h.htgvzlr3zeg1"></a>3 | <a name="h.c9zuyf785t1k"></a>4 | <a name="h.atfwyo73js4d"></a>5 | <a name="h.h7dqlwqgya0e"></a>6 | <a name="h.6ekecfdp08zm"></a>7 | <a name="h.emgt12bv0g4g"></a>8 | <a name="h.cmpkt9yv0iat"></a>9 | <a name="h.je7t7q6tv1rf"></a>10 |
<a name="h.s8aq2lbk47y3"></a>DNA | <a name="h.rwp11tgj4ahh"></a>1 | <a name="h.z2tat31a3gar"></a>1 | <a name="h.i98vrbkqsqnm"></a>1 | <a name="h.usa99qhtlxql"></a>1 | <a name="h.yk37pz4wnolg"></a>1 | <a name="h.93mvjlyrb5jn"></a>1 | <a name="h.dz2ctq9w2aqk"></a>1 | <a name="h.sotzy92rv1s"></a>1 | <a name="h.li3a7e2bdvn1"></a>1 | <a name="h.t6ryvfn516e3"></a>1 |
<a name="h.e6r7lvtrxib4"></a>F | <a name="h.9c8jtdfzcapw"></a>1 | <a name="h.glq9z4l3ebu"></a>1 | <a name="h.cy5bp9k13ic0"></a>1 | <a name="h.9d7e08bmzv1d"></a>1 | <a name="h.gduu3qbma75b"></a>1 | <a name="h.z6t16hk7if5r"></a>1 | <a name="h.39dyx564wgqh"></a>1 | <a name="h.40oje46pms7d"></a>1 | <a name="h.np1vcv5u8g5k"></a>1 | <a name="h.emqtdc1e10t6"></a>1 |
<a name="h.azwizg70184b"></a>R | <a name="h.o5hmje4g5c8u"></a>1 | <a name="h.ukf167shdgjo"></a>1 | <a name="h.o41h7oavaiam"></a>1 | <a name="h.tf8kg14l1y3r"></a>1 | <a name="h.irnniwmsayy0"></a>1 | <a name="h.ygpgl6h0jhre"></a>1 | <a name="h.i71phhtxr5lq"></a>1 | <a name="h.o1o13ry40x9f"></a>1 | <a name="h.752xc4awlv9t"></a>1 | <a name="h.s82d9wj9hh0f"></a>1 |
<a name="h.wwgb8rb2caqx"></a>Phusion | <a name="h.ot6w07vgsmho"></a>10 | <a name="h.j0qp0j8yylz"></a>10 | <a name="h.9ka3k0y4zl04"></a>10 | <a name="h.tf6hvlrn1u79"></a>10 | <a name="h.pnybwmk1amlp"></a>10 | <a name="h.vyeifkgxpagy"></a>10 | <a name="h.vy8zdbxwo69v"></a>10 | <a name="h.13lc0p7rp4g"></a>10 | <a name="h.9rvgw4inrhql"></a>10 | <a name="h.bgvpr1ym61fz"></a>10 |
<a name="h.zfb5f4i9rdii"></a>Water | <a name="h.sta2t5jqzrvw"></a>7 | <a name="h.icutzgtuflsq"></a>7 | <a name="h.dvu38r3f8g1t"></a>7 | <a name="h.62umehxctlvk"></a>7 | <a name="h.1zld32o1mbx4"></a>7 | <a name="h.q7dclgac2akm"></a>7 | <a name="h.e53dqcg8lwdh"></a>7 | <a name="h.thms7q28qo7y"></a>7 | <a name="h.5hwyopy6g198"></a>7 | <a name="h.h268jsv0xfi7"></a>7 |
<a name="h.ptzum1ut187j"></a>
<a name="h.625habuoaedf"></a>Digestion
<a name="h.aksdanvknykf"></a>
<a href="#" name="c5f20c3bfc7fb1e9388cd5cdff6e1ead54d8a697"></a><a href="#" name="10"></a><a name="h.537gplby40eo"></a> | <a name="h.xqc0lppe6krv"></a>5 (i5-13-9) | <a name="h.ubh03cwp1deq"></a>6 (i3-13-10) | <a name="h.o2wbmaardiau"></a>7 (i3-13-11) | <a name="h.ovwu3kvq8nyk"></a>8 (i3-13-12) | <a name="h.y6s4vn5x3iav"></a>9 (i3-13-13) | <a name="h.smdjo587yn7d"></a>i5-10-3 (i5-13-14) |
<a name="h.xntrywweu8of"></a>DNA | <a name="h.lv3064b3lmj"></a>30 | <a name="h.8ph34t10kt1k"></a>30 | <a name="h.rsoug65yaof9"></a>30 | <a name="h.er67n63cg2v7"></a>30 | <a name="h.bqbab170861d"></a>30 | <a name="h.igemoh6tuopz"></a>30 |
<a name="h.3z2okkavkzgn"></a>NE Buffer | <a name="h.23pmrd3j3kfz"></a>5 | <a name="h.vsrsr7pdqtt0"></a>5 | <a name="h.epa3h1153om3"></a>5 | <a name="h.4ikn46vluxyw"></a>5 | <a name="h.gxe844eltiua"></a>5 | <a name="h.iaz8nua1eyfu"></a>5 |
<a name="h.2uuz4e40isx1"></a>EcoRI | <a name="h.i0xp596p5fw1"></a>1 | <a name="h.sqlcqmgvj173"></a>1 | <a name="h.cq48ztpsv71d"></a>1 | <a name="h.ck15m3arqdrd"></a>1 | <a name="h.93tkn3hqbi9w"></a>1 | <a name="h.w0glvf9ljf32"></a>1 |
<a name="h.77qgyz3qlwhs"></a>Prst I | <a name="h.dotv3dly1w9n"></a>1 | <a name="h.m0cauz10idxo"></a>1 | <a name="h.pzaip3q8vyky"></a>1 | <a name="h.5ccfp79x0sya"></a>1 | <a name="h.p1vfow1jids1"></a>1 | <a name="h.mq5gkij39x1m"></a>1 |
<a name="h.tofmw9c4te4v"></a>Water | <a name="h.6dwq0bt714dx"></a>13 | <a name="h.t6qsxr11k62i"></a>13 | <a name="h.dbq1oskgtj8a"></a>13 | <a name="h.j6u49lawj21h"></a>13 | <a name="h.loge284qwxle"></a>13 | <a name="h.vmjjkyo4l4vt"></a>13 |
<a name="h.kvb5pahdagn6"></a>7/24/2013
<a name="h.d8tsh5kkz4h0"></a>PCR
<a name="h.hskqvv6hw324"></a>
<a href="#" name="a83db890a01d761808d0df4a6f54456aee0869c6"></a><a href="#" name="11"></a><a name="h.q47n76xr18ri"></a>Primers | <a name="h.7rkpqmb3uwac"></a>1 | <a name="h.8kvhh4ls95g1"></a>2 | <a name="h.l6ktdui81p2i"></a>3 | <a name="h.wg2w0swmphbf"></a>4 |
<a name="h.aupmsss8kqaz"></a>DNA | <a name="h.us5i9ie2ief7"></a>1 | <a name="h.d1arncc0oq0i"></a>1 | <a name="h.wx2lt1gmgejp"></a>1 | <a name="h.moqlznfx8kxv"></a>1 |
<a name="h.ea6xpevky1n5"></a>R | <a name="h.2lo40l8nn351"></a>1 | <a name="h.c4nvgln2gort"></a>1 | <a name="h.l4ns7ijsrf9a"></a>1 | <a name="h.v67x0t1gynjr"></a>1 |
<a name="h.g728ofbrsyd2"></a>F | <a name="h.log4kdfc2xut"></a>1 | <a name="h.2tilhu3yk376"></a>1 | <a name="h.mrnv1jd0m6vk"></a>1 | <a name="h.19fqyxi4lj5b"></a>1 |
<a name="h.wgb3khr5skw2"></a>Phusion | <a name="h.63er7ixlb9ud"></a>10 | <a name="h.3mxustbm97sx"></a>10 | <a name="h.katv3qd44lkw"></a>10 | <a name="h.q7gjuhvs0um"></a>10 |
<a name="h.4hdp70vtlra"></a>Water | <a name="h.sndqj4z3vcvl"></a>7 | <a name="h.1f065lbzz1z"></a>7 | <a name="h.gvon0ul75w87"></a>7 | <a name="h.xoffqy49dsnd"></a>7 |
<a name="h.vw3bshxwwuxf"></a>
<a name="h.koawdqsmnyoh"></a>1 i5-14-1 33.3 ng/ul (Com C)
<a name="h.fv1vt78mg7ru"></a>2 i5-14-2 47.5 ng/ul (Com D)
<a name="h.3x1x0benh4pe"></a>3 i5-14-3 38.4 ng/ul (Com E)
<a name="h.4z3kd4o6avp1"></a>4 i5-14-4 27.8 ng/ul (Com A)
<a name="h.l08jj5jvxhhm"></a>
<a name="h.byev1ekaa63a"></a>7/30/2013
<a name="h.hhq1lr5p0okp"></a>Digestion
<a name="h.rz8lt1ph5jxn"></a>1 (i5-14-1) (Com C)
<a name="h.n0uh9rz1mf4q"></a>DNA 30 (0 left)
<a name="h.kneof781dyz1"></a>NEBuffer 5
<a name="h.i4qtg9crqsm6"></a>EcoRI 1
<a name="h.db59aiebk0lj"></a>PstI 1
<a name="h.fw9odm6ololp"></a>Water 13
<a name="h.ut30mxfqsmhf"></a>→ new i5-14-1
<a name="h.2flutjvnrfl1"></a>
<a name="h.qyltrisqlr82"></a>Gel extraction i5-14-1
<a name="h.lag2kt5dnjgh"></a>
<a name="h.mvk988hr22kv"></a>i5-13-9 (Com B) 8.6 ng/ul
<a name="h.dj3ugc0biey"></a>i5-13-10 (ScrR) 12 ng/ul
<a name="h.5qit7p46px2g"></a>i513-11(pScr) 14.4 ng/ul
<a name="h.3do6oe7lvk2u"></a>i5-13-12 (Cra) 15.1 ng/ul
<a name="h.5y516xtktq34"></a>i5-13-13(pFru) 11 ng/ul
<a name="h.4boqh7b34zdl"></a>i5-13-14(Chlora backbone) 24 ng/ul
<a name="h.s5lp4mbltzhy"></a>i5-15-1 (Com C) 13.9 ng/ul
<a name="h.8r9vz5eadw0t"></a>
<a name="h.ut6ozfvulmxz"></a>Ligation
<a name="h.pfehlplbqkke"></a>Vector i5-13-14 (Chlora)
<a name="h.8r7mn68yco2x"></a>
<a name="h.i07o62sb0skt"></a>
<a href="#" name="50d13a574052472cd642da77fe9561faf1a141d9"></a><a href="#" name="12"></a><a name="h.rwclbd7ju5o6"></a>Insert: | <a name="h.eapxxn7uc1g6"></a>i5-13-9 | <a name="h.8yh49v9ynpyf"></a>i5-13-9 | <a name="h.z9qy3zix2fy9"></a>i5-13-10 | <a name="h.pjoqdlvv3lkm"></a>i5-13-10 | <a name="h.wq1gnmk3rbzf"></a>i5-13-11 | <a name="h.n8s28blj6ch8"></a>i5-13-11 | <a name="h.vzyxdfb5t3sd"></a>i5-13-12 | <a name="h.2qrdexvawwpk"></a>i5-13-12 | <a name="h.z6v5lw64ahad"></a>i5-13-13 | <a name="h.y5i9fqvu88i6"></a>i5-13-13 | <a name="h.ucy77na90940"></a>i5-15-1 | <a name="h.n4drv21h8wzz"></a>i5-15-1 |
<a name="h.ddozs2852nhp"></a> | <a name="h.4v3arya9n04w"></a>3:1 | <a name="h.lze3kxcsc4ks"></a>0:1 | <a name="h.7gpyhvx1ddwn"></a>3:1 | <a name="h.tb20iaswej"></a>0:1 | <a name="h.hf9fz1ks5wzs"></a>3:1 | <a name="h.yvfgmo49urdc"></a>0:1 | <a name="h.xbpyfe5ghwhd"></a>3:1 | <a name="h.s365vqt413uu"></a>0:1 | <a name="h.tj1zd9fyq725"></a>3:1 | <a name="h.gv8xkigvkbxs"></a>0:1 | <a name="h.bn78bbm9lzz5"></a>3:1 | <a name="h.11iltw2g7gja"></a>0:1 |
<a name="h.8xwue3hhxoj2"></a>Vector | <a name="h.ueapcumgr70z"></a>2 | <a name="h.a90678jyza68"></a>2 | <a name="h.e6e899jjwqh"></a>2 | <a name="h.v9uy9dru9lha"></a>2 | <a name="h.7s651ehvei4b"></a>2 | <a name="h.i107go106z3z"></a>2 | <a name="h.xhtik8jfr5t8"></a>2 | <a name="h.7jycjrl59rkh"></a>2 | <a name="h.bw4ew4c4843j"></a>2 | <a name="h.1yf4govsdmmz"></a>2 | <a name="h.dy0o6cu8osv8"></a>2 | <a name="h.e1bg38dvm6ts"></a>2 |
<a name="h.cykgsr2weglr"></a>insert | <a name="h.eizukidl4cyc"></a>7.5 | <a name="h.kog90q6bx275"></a>0 | <a name="h.98vnva3vgr2u"></a>6 | <a name="h.9u7gxwmfqfpt"></a>0 | <a name="h.azeiwoyo9gg3"></a>1 | <a name="h.shdnosdzcw16"></a>0 | <a name="h.xhdyltdzx6ea"></a>5 | <a name="h.qb978w6jidz"></a>0 | <a name="h.mdvwwm8w3gr2"></a>1.5 | <a name="h.berxsw3ctoj9"></a>0 | <a name="h.bws8wghda8eq"></a>1 | <a name="h.exx4y5x68751"></a>0 |
<a name="h.ufw4a7mv08qf"></a>buffer | <a name="h.e82ys14lie5g"></a>2 | <a name="h.w3awm2jc8x62"></a>2 | <a name="h.ip79buro7z35"></a>2 | <a name="h.2jwvv8pad0y8"></a>2 | <a name="h.eui2glumbma9"></a>2 | <a name="h.ij6a4wggvhr8"></a>2 | <a name="h.5erup0qxiaoa"></a>2 | <a name="h.34drqu9lsiip"></a>2 | <a name="h.sxt14q53zna"></a>2 | <a name="h.zeib64cg79d2"></a>2 | <a name="h.w76ejfa0vgam"></a>2 | <a name="h.sli6mh394kb5"></a>2 |
<a name="h.v35ueq5cr2ki"></a>ligase | <a name="h.jp2k3gq27m9p"></a>1 | <a name="h.l2ezm01epi51"></a>1 | <a name="h.ep4rxobcrnm0"></a>1 | <a name="h.gr1lj3ltfo95"></a>1 | <a name="h.gf8vy915vn"></a>1 | <a name="h.trcwr9gqzc95"></a>1 | <a name="h.ap4irlbntl3b"></a>1 | <a name="h.brtf4pa8s3tg"></a>1 | <a name="h.56a01qf31wqd"></a>1 | <a name="h.8nysqqn9xpga"></a>1 | <a name="h.cgwytf5eesu9"></a>1 | <a name="h.b39j5p6tch1k"></a>1 |
<a name="h.kf7kmjciwkpj"></a>water | <a name="h.l5mxf8eibg1"></a>7.5 | <a name="h.4hvglti0oxpi"></a>15 | <a name="h.jr1bst19a9wz"></a>9 | <a name="h.wzkvjibfhcnb"></a>15 | <a name="h.pndyfze6qn5i"></a>14 | <a name="h.gub9i3ohcehi"></a>15 | <a name="h.i2kexdv8pq33"></a>10 | <a name="h.o7nzrkza94cd"></a>15 | <a name="h.xpe8r41l59s0"></a>13.5 | <a name="h.55gekl6q1m72"></a>15 | <a name="h.ym8dfmbzpnfk"></a>14 | <a name="h.1nuuijugz687"></a>15 |
<a name="h.xt2svhv3b8l4"></a>total | <a name="h.2tn6w1uqmqxz"></a>20 | <a name="h.sdpcuzjyl7ts"></a>20 | <a name="h.ourvjjokdadc"></a>20 | <a name="h.bsdbvy1o7wln"></a>20 | <a name="h.jwaa3xuxas9"></a>20 | <a name="h.hqmcbdgpw0lx"></a>20 | <a name="h.e0ehwrwd4agz"></a>20 | <a name="h.zfiuqf321edu"></a>20 | <a name="h.ps0ewmqtqv9o"></a>20 | <a name="h.n6pyhtm59dbi"></a>20 | <a name="h.y4lt0enlcu3u"></a>20 | <a name="h.riegvhuvgl4n"></a>20 |
<a name="h.abfr1wtzld8l"></a> | <a name="h.eue2n4kohh0l"></a>1 | <a name="h.ef50nw1y7qha"></a>2 | <a name="h.2l6f7excv42h"></a>3 | <a name="h.3c6fmyyunrm7"></a>4 | <a name="h.l5ht1kgx2r3d"></a>5 | <a name="h.zi9axt96eqt2"></a>6 | <a name="h.qr6e4yv2ily7"></a>7 | <a name="h.exxb2fxp0zbw"></a>8 | <a name="h.kuz4x1yluh03"></a>9 | <a name="h.7luzyy8w7tqz"></a>10 | <a name="h.wzwxx8s2jhfe"></a>11 | <a name="h.c4dyb9ecvegy"></a>12 |
<a name="h.gfculfarh7ou"></a>
<a name="h.mhtosyfxiplo"></a>Transformation
<a name="h.i3wtjhdwzin3"></a>1
<a name="h.yqiuna5qzuv"></a>2
<a name="h.6506ohlh2qwn"></a>3
<a name="h.ic0zbvoiwg54"></a>4
<a name="h.7jw4qtpuq8u"></a>5
<a name="h.vxoc92u740ec"></a>6
<a name="h.xw9bu3ftnlrz"></a>7
<a name="h.wz32he1cowmw"></a>8
<a name="h.xu12aug1fjsk"></a>9
<a name="h.6nxy36gth9ap"></a>10
<a name="h.6vj90p5kdqyh"></a>11
<a name="h.2ivfpv951rq5"></a>12
<a name="h.xrfzapxgozv0"></a>+ control → i5-10-4
<a name="h.a30bxd2gr253"></a>
<a name="h.2sye30drygjv"></a>Inconsistent growth (repeat)
<a name="h.rcwo2mwhmsj8"></a>
<a name="h.qdwpmlar9fxp"></a>7/30
<a name="h.h2yrqpht6uek"></a>Transformation
<a name="h.g16bsupb0if7"></a>1
<a name="h.yepbrmq46j8j"></a>2
<a name="h.yuzf97e1ycl1"></a>3
<a name="h.4k2gx4fcrj7c"></a>4
<a name="h.ytz71xaxz9i"></a>5
<a name="h.y9wrgq1sinlr"></a>6
<a name="h.f4nyxt67c1g6"></a>7
<a name="h.chgf8ey2p72w"></a>8
<a name="h.6yor6rdyrcfh"></a>9
<a name="h.ysyhpy20dxld"></a>10
<a name="h.wgqbqis3fu9k"></a>11
<a name="h.g2va1il9rme3"></a>12
<a name="h.va67wmeyjr7z"></a>+ control → i5-10-4
<a name="h.l3gyqob9ad8r"></a>
<a name="h.lyf956klvtnq"></a>Inoculate
<a name="h.cu4kdzhfgn9j"></a>From glycerol stock i5-11-5 (pLac-GFP)
<a name="h.cvxy2odcnx2"></a>100.4 ng/ul
<a name="h.72910tlji9sm"></a>96.4 ng/ul [pLac-GFP]
<a name="h.gxyw4fotd64k"></a>84.3 ng/ul
<a name="h.blxr8wovn17y"></a>
<a name="h.1sjg3n4ze7nq"></a>7/31
<a name="h.iwchhdbervw9"></a>Transformation Results
<a name="h.5atic82knicq"></a>1 [Com B] → 8 colonies → Picked 3 colonies→ i5-16-1
<a name="h.k4hslldhwd8r"></a> i5-16-2
<a name="h.16gg4u89efie"></a> i5-16-3
<a name="h.9m4le8otw8qu"></a>2→ 50 colonies
<a name="h.tbck2neavtxc"></a>3 [Scr] → 11 colonies → Picked 3 colonies → i5-16-4
<a name="h.orwfvyt5ue2v"></a> i5-16-5
<a name="h.242xprdzg0b5"></a> i5-16-6
<a name="h.8faqzlhxo0iq"></a>4 → 20 colonies
<a name="h.ds7rd5ef53tw"></a>5 [pScr] → 25 colonies → picked 3 colonies → i5-16-7
<a name="h.uninw76k2wf"></a> i5-16-8
<a name="h.qpdj9aujpd2t"></a> i5-16-9
<a name="h.405vih9kuvu"></a>6 → 0 colonies
<a name="h.ox6mwhqz0f0f"></a>7 [Cra] → 50+ colonies → picked 3 colonies → i5-16-10
<a name="h.3pqoz5468f0a"></a> i5-16-11
<a name="h.g56vdta42xvu"></a> i5-16-12
<a name="h.uf67ey9w9f40"></a>8 → 75+ colonies
<a name="h.aoggldrolu7c"></a>9 [pFru] → 75+ colonies → picked 3 colonies → i5-16-13
<a name="h.z6au5v2jb975"></a> i5-16-14
<a name="h.90uwnto7k70s"></a> i5-16-15
<a name="h.n89z936984bi"></a>10 → 11 colonies
<a name="h.283avhppju1z"></a>11 [Com C] → 30 colonies → picked 3 colonies→ i5-16-16
<a name="h.92qdy4j90bwo"></a> i5-16-17
<a name="h.wsv606ce7ery"></a> i5-16-18
<a name="h.ubmjrqntcnja"></a>12→ 30 colonies
<a name="h.mj4433clon1w"></a>13→ 7 colonies
<a name="h.u0ea7xjc6274"></a>14 →2 colonies
<a name="h.d9vx3nyuimz7"></a>15 → 20 colonies
<a name="h.f2cq7ihd00rt"></a>16→ 10 colonies
<a name="h.9cfq1743wgqc"></a>17 → 0 colonies
<a name="h.okzp12kpgfhd"></a>18 → 5 colonies
<a name="h.m6j8vq7xdy0l"></a>+ control → ∞ colonies
